Gene amplification

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide

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435 91, 4352401, 4352402, 4351721, 4351723, 435243, 435320, 536 27, 935 6, 935 34, 935 42, 935 43, 935 61, 935 70, C12P 2100, C12P 1934, C12N 500, C12N 1500, C12N 100, C07H 2104

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047660678

ABSTRACT:
Hybrid DNA capable of effecting controlled amplification in a host cell of a heterologous gene that determines a specific function. The hybrid DNA has the following three different regions of DNA: (1) an "amplicon" that is inducible by an external stimulus; (2) the heterologous gene (or a site for its insertion); and (3) a selectable marker enabling isolation of the transformed host cells. In order to make a desired compound coded by the heterologous gene, a vehicle including the hybrid DNA is introduced into a host cell is transformed with the vector, transformed host cells are grown to a desired population, the external stimulus is controlled to effect amplification of the gene in members of the population, cells containing amplified gene are cultured, and the compound is recovered.

REFERENCES:
patent: 4656134 (1987-04-01), Ringold
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D. K. Biswas et al., "Bromodeoxyuridine Induced Amplification of Prolactin Gene in GH Cells", Advances in Gene Technology, Proceedings of the 19th Miami Winter Symposium, Feb. 1987.
D. J. Wilson et al., "Bromodeoxyuridine-Induced Amplification of Prolactin Gene in a GH Subclone," Abstracts 22nd Annual Meeting American Society for Microbiology.
D. K. Biswas et al., "Bromodeoxyuridine Induced Amplification of Prolactin Gene in GH Cells is an Extrachromosomal Event", 74th Annual Meeting of Biological Chemists, Jun. 1983, Federation Proceedings.
Stark and Wahl (1984) Gene Amplification, Ann. Rev. Biochem. 53:447-491.
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Biswas and Hanes (1982) Nucleic Acid Res. 10:3995-4008.
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Harvard Medical Area Focus, Jan. 12, 1984, pp. 1, 7 and 8.
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