Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1985-05-31
1988-08-23
Martinell, James
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
435 91, 4352401, 4352402, 4351721, 4351723, 435243, 435320, 536 27, 935 6, 935 34, 935 42, 935 43, 935 61, 935 70, C12P 2100, C12P 1934, C12N 500, C12N 1500, C12N 100, C07H 2104
Patent
active
047660678
ABSTRACT:
Hybrid DNA capable of effecting controlled amplification in a host cell of a heterologous gene that determines a specific function. The hybrid DNA has the following three different regions of DNA: (1) an "amplicon" that is inducible by an external stimulus; (2) the heterologous gene (or a site for its insertion); and (3) a selectable marker enabling isolation of the transformed host cells. In order to make a desired compound coded by the heterologous gene, a vehicle including the hybrid DNA is introduced into a host cell is transformed with the vector, transformed host cells are grown to a desired population, the external stimulus is controlled to effect amplification of the gene in members of the population, cells containing amplified gene are cultured, and the compound is recovered.
REFERENCES:
patent: 4656134 (1987-04-01), Ringold
Ringold et al, J. Molec. Appl. Genet. 1: 165 (1981).
D. K. Biswas et al., "Bromodeoxyuridine Induced Amplification of Prolactin Gene in GH Cells", Advances in Gene Technology, Proceedings of the 19th Miami Winter Symposium, Feb. 1987.
D. J. Wilson et al., "Bromodeoxyuridine-Induced Amplification of Prolactin Gene in a GH Subclone," Abstracts 22nd Annual Meeting American Society for Microbiology.
D. K. Biswas et al., "Bromodeoxyuridine Induced Amplification of Prolactin Gene in GH Cells is an Extrachromosomal Event", 74th Annual Meeting of Biological Chemists, Jun. 1983, Federation Proceedings.
Stark and Wahl (1984) Gene Amplification, Ann. Rev. Biochem. 53:447-491.
Schimke et al. (1978) Science 202:1051-1055.
Wahl et al. (1979) J. Biol. Chem. 254:8679-8689.
Biswas and Hanes (1982) Nucleic Acid Res. 10:3995-4008.
Biswas et al. (1977) Cell 11:431-439.
Wilson et al. (1983) DNA 2:237-242.
Harvard Medical Area Focus, Jan. 12, 1984, pp. 1, 7 and 8.
Chien and Thompson (1980) Proc. Natl. Acad. Sci. USA 77:4583-4587.
Enquist et al. (1979) Gene 7:335-342.
Biswas et al. (1984) Science 219:941-943.
Martinell James
President and Fellows of Harvard College
LandOfFree
Gene amplification does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Gene amplification, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Gene amplification will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-835622