Geminivirus-based gene expression system

Details Geminivirus-based gene expression system Geminivirus-based gene expression system

1994-05-23

1996-12-31

Fox, David T.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435 691, 4351723, 4352351, 4353201, 536 2372, C12N 504, C12N 1534, C12N 1582, C12N 1583, C12N 1584

Patent

active

055893791

ABSTRACT:
A geminivirus based vector system for obtaining controlled expression of a nucleic acid fragment of interest is disclosed. Tissue specific regulatory regions are identified employing cDNA screening and the resulting tissue--specific regulatory regions are manipulated for use in geminivirus constructs to provide for transcription and/or expression of nucleic acid sequences nonindigenous to the geminivirus vector for introduction into plant cells. The vector system may be used to provide transformed plants having cells, tissues or parts with a modified phenotypic property.

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Sunter, et. al "Transactivation of Geminivirus AR1 and BR1 Gene Expression by the Viral AL2 Gene Product Occurs at the Level of Transcription" The Plant Cell (Oct. 1992) vol. 4, No. 10 1321-1331.

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