Gelling of pectic material using carboxylic ester hydrolase and

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

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435 72, C12P 1904, C12P 1900

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active

059981768

ABSTRACT:
A method for causing gelling or increase of viscosity of an aqueous medium containing a gellable polymeric material which has functionalities with phenolic hydroxy groups, and which, in aqueous medium, is susceptible to viscosity increase or gelling in the presence of a carboxylic ester hydrolase, comprises treating the aqueous medium with: a carboxylic ester hydrolase (EC 3.1.1); and an oxidase (EC 1.10.3) and/or a peroxidase (EC 1.11.1); in the presence of an oxidizing agent suitable for use with the oxidase and/or peroxidase. Gelled products obtainable by the method may be dried or dehydrated to give products which are useful as absorbents for absorbing aqueous media, such as body fluids. In particular, gelling a pectic material obtainable from sugar beets using pectinesterase (EC 3.1.1.11) and laccase (EC 1.10.3.2).

REFERENCES:
patent: 4672034 (1987-06-01), Rombouts
patent: 5443975 (1995-08-01), Cervelli et al.
Milstein et al., "Transformation of lignin-related compounds with laccase in organic solvents", Journal of Biotechnology, 1993, vol. 30, pp. 37-47.
Rombouts et al., Enzymatic and chemical degradation and the fine structure of pectins from sugar-beet pulp, Carbohydrate Research, 1986, vol. 154, pp. 189-203.
Williamson et al., "Gelation of sugarbeet and citrus pectins using enzymes extracted from orange peel", Carbohydra. Polym., 1990, 13(4), pp. 387-397.
Guillon et al., "Oxidative cross-linking of chemically and enzymatically modified sugar-beet pectin", Carbohydr. Polym., 1990, 12(4), pp. 352-374.

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