Gel product for separation purposes and method of using the prod

Details Gel product for separation purposes and method of using the prod Gel product for separation purposes and method of using the prod

1974-03-19

1976-06-01

Adee, John

Chemistry of carbon compounds

Miscellaneous organic carbon compounds

C-metal

55386, 210198C, 210502, 252316, 252426, 210 24, 210 31C, B01D 1508

Patent

active

039607208

ABSTRACT:
The invention concerns a gel product for chromatography based on agarose, and a method of using the product for hydrophobe salting out adsorption. The gel product is characterized in that the hydrophobe groups are bonded to the agarose with ether or esther binding, and that the hydrophobe groups can be composed of aliphatic hydrocarbons, halogen or hydroxy-substituted alkyl groups, with 1 to 20 carbon atoms, or contain 1 or more, up to 5, aromatic nuclei, unsubstituted or substituted with alkyl, alkyloxy, halogen or NO.sub.2 or have the formula ##EQU1## where R can be alkyl, alkenyl, aryl, aralkyl, aralkoxy, alkaryl, optionally substituted with hydroxy, halogen or nitro, and where the alkyl parts of R contain 1 to 20 carbon atoms. The method of separating the amphipatic substances is based on the fact that they are adsorbed by an amphipatic gel in which the salt concentration in the solution is adjusted beyond the level at which the ion concentration least corresponds to the ion concentration in an aqeous solution of 1 M NaCl, and desorption takes place through lowering the ion concentration and/or lowering the pH and/or lowering the polarity of the solvent. The amphipatic substances are primarily proteins or peptides.

REFERENCES:
patent: 3002823 (1961-10-01), Florin et al.
patent: 3285849 (1966-11-01), Watanabe et al.
patent: 3350174 (1967-10-01), Mattenheimer
patent: 3453257 (1969-07-01), Parmerter et al.
patent: 3527712 (1970-09-01), Renn et al.
patent: 3536614 (1970-10-01), Faisque et al.
patent: 3787317 (1974-01-01), Jaworek
patent: 3850798 (1974-11-01), Sjoquist

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