Gel plates, equipment and kits for combined electrophoretic-immu

Chemical apparatus and process disinfecting – deodorizing – preser – Analyzer – structured indicator – or manipulative laboratory... – Sample mechanical transport means in or for automated...

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

422101, 422104, 204299R, 436516, B01D 6142, B01D 5702

Patent

active

052386511

ABSTRACT:
Combined electrophoretic-immunoelectrophoretic analysis is conducted using a gel plate having a plurality of preformed trenches therein dividing the plate into a number of electrophoretic tracks. Serum samples are applied to the tracks such that each pair of tracks has the same sample. After subjecting the tracks to electrophoresis, one track from each pair of matching tracks is removed for protein staining by inserting a spatula into a trench and under the track to be removed. Small dabs of high vacuum silicone stopcock grease are applied to the ends of each sidewall of the remaining gel tracks and antiserum is applied therebetween. After immunoprecipitation and staining, the removed tracks are returned to their original positions to permit exact correlation of said immunoprecipitation and electrophoresis patterns. This analysis technique may be facilitated by use of equipment which includes a plate holder, a plate holder frame, underlay inserts, left and right margin inserts, a guide bar frame, templates, spatulas and plate handling hooks, burnishing rods and applicator pens. The trenches may be formed in conventional gel plates by specially designed equipment including a trench former insert frame, a double bladed gel cutter and a gel displacement beak.

REFERENCES:
patent: 3499360 (1968-02-01), Davis
patent: 3635808 (1972-01-01), Elevitch
patent: 3844918 (1974-10-01), Cawley
patent: 3988230 (1976-10-01), Krotz
patent: 4094759 (1978-06-01), Ruhenstroth-Bauer et al.
patent: 4246222 (1981-01-01), Monthony
patent: 4312727 (1982-01-01), Shainoff
patent: 4321119 (1982-03-01), Ambler
patent: 4329213 (1982-05-01), Elwing
patent: 4337131 (1982-06-01), Vesterberg
patent: 4385974 (1983-05-01), Shevitz
patent: 4417967 (1983-11-01), Ledley
patent: 4452901 (1984-06-01), Gordon et al.
patent: 4558007 (1985-12-01), Anderson et al.
patent: 4575484 (1986-03-01), Straus
patent: 4599305 (1986-07-01), Witte et al.
patent: 4668363 (1987-05-01), Gebott et al.
patent: 4713349 (1987-12-01), Levin
patent: 4891319 (1990-01-01), Roser
patent: 4954236 (1990-09-01), Kushner et al.
patent: 4970070 (1990-11-01), Raft
Karinkanta, H. H.; Nieminem, E. J.; An Improved Technique for Immunofixation of Electrophoretograms, Clin. Chem., 24/9, 1639-41 (1978).
Life Technologies Inc., Catalogue and Reference Guide, 1989.
Chuba, "Microimmunoelectrophoresis with Improved Resolution", Journal of Applied Biochemistry, vol. 1, pp. 37-50 (1979).
Poulik, "Filter Paper Electrophoresis of Purified Diphtheria Toxoid", Can. J. Med. Sci., 30:417-419 (1952).
Kohn, "An Immuno-electrophoretic Technique", Nature, vol. 180, 986-987 (1957).
Pizzolato et al., "Immunofixation on Cellulose Acetate: An Improved Screening Method for Monoclonal Immunoglobulins", Journal of Immunological Methods, 26:365-368 (1979).
Alper et al., "Immunofixation Electrophresis: A Technique for the Study of Protein Polymorphism", Vox Sang, 17:445-452 (1969).
Grabar et al., "Methode Permettant L'Etude Conjuguee Des Proprietes Electrophoretiques et Immunochimiques D'Un Melange De Proteines, Application au Serum Sanguin", Biochem. Biophys. Acta., vol. 10, 193-194 (1953).
Scheidegger, "Une micro-methode de l'immuno-electrophorese", Int. Arch. Allergy. Appl. Immunolol., vol. 7, pp. 103-110 (1955).
Elek, "The Serological Analysis of Mixed Flocculating System by Means of Diffusion Gradients", British Journal of Experimental Pathology, 2:484-500 (1949).
Saber et al., "The Role of IFE in Identifying and Quantitating Immunoglobulins", Clinical Lab Products, 14(1): 21-27 (1985).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Gel plates, equipment and kits for combined electrophoretic-immu does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Gel plates, equipment and kits for combined electrophoretic-immu, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Gel plates, equipment and kits for combined electrophoretic-immu will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-826994

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.