Gel electrophoresis using time dependent contour controlled elec

Details Gel electrophoresis using time dependent contour controlled elec Gel electrophoresis using time dependent contour controlled elec

1988-03-21

1992-01-28

Niebling, John F.

Chemistry: electrical and wave energy

Apparatus

Coating, forming or etching by sputtering

G01N 2726, B01D 5702

Patent

active

050841572

ABSTRACT:
An apparatus for separating particles within a medium which is capable of generating electric fields having adjustable magnitudes and orientations is disclosed herein. The electrophoretic apparatus of the present invention includes electrode arrangement for providing a plurality of sets of opposing individually responsive electrodes for generating electric fields within the medium, each field having at least one magnitude and direction. The invention further includes a control circuit for selectively controlling electrodes within the electrode sets to vary the magnitudes and directions of the electric fields at locations within the medium. The electric fields induce differing rates of motion of the particles thereby separating the particles.

REFERENCES:
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patent: 4159523 (1979-06-01), Neer
patent: 4320415 (1982-03-01), Jones
patent: 4473452 (1984-09-01), Cantor
patent: 4674323 (1987-06-01), Rulf
patent: 4737251 (1988-04-01), Carle et al.
McPeek, Jr. F. D., et al. "Separation of Large DNA Molecules by Modified Pulsed Field Gradient Gel Electrophoresis"Analytical Biochemistry, 156, pp. 274-285 (1986).
Chu et al., "Separation of Large DNA Molecules by Contour-Clamped Homogenous Electric Fields", Science, vol. 234, p.1583 (1986).
Ellis et al., "Ramped Field Inversion Gel Electrophoresis: A Cautionary Note", Nucleic Acids Research, vol. 15, p. 5489 (1987).

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