Gel cassette for electrophoresis

Details Gel cassette for electrophoresis Gel cassette for electrophoresis

1998-02-23

2000-02-22

Warden, Jill

Chemistry: electrical and wave energy

Apparatus

Electrophoretic or electro-osmotic apparatus

204461, 204466, 204467, 204612, 204616, G01N 2726, G01K 27447

Patent

active

060276282

ABSTRACT:
A gel cassette for electrophoresis includes opposed plastic plates holding a gel therebetween. An upper buffer tank can be adhered to one of the plates by double backed tape that is sandwiched between the tank and plate, thereby avoiding the use of a rubber sealing gasket between the tank and plate. As a result, the distance between the tank and the plate is kept constant. A buffer solution in the tank communicates with the gel. The cassette can then be placed in a lower buffer tank and an electric field applied to the buffer solutions to effect electrophoresis of DNA fragments that have been placed in the gel. In an alternate embodiment, a window is formed in one of the plastic plates to allow laser light to pass into the cassette and excite a fluorescent tag in the DNA. The fluorescence then passes back through the window for detection by a sensor, to automatically determine the DNA sequence of the sample in the gel in accordance with auto DNA sequencing principles. The window reduces and indeed eliminates absorption, interference, and/or reflection of the relatively weak fluorescent signal emitted by the DNA tag.

REFERENCES:
patent: 3129158 (1964-04-01), Raymond et al.
patent: 3442686 (1969-05-01), Jones
patent: 3932263 (1976-01-01), Brefka
patent: 4548869 (1985-10-01), Ogawa et al.
patent: 4548870 (1985-10-01), Ogawa et al.
patent: 4576693 (1986-03-01), Kreisher et al.
patent: 4579783 (1986-04-01), Ogawa et al.
patent: 4600641 (1986-07-01), Ogawa et al.
patent: 4718998 (1988-01-01), Ogawa et al.
patent: 4722777 (1988-02-01), Ogawa et al.
patent: 4729823 (1988-03-01), Guevara, Jr.
patent: 4737258 (1988-04-01), Ogawa et al.
patent: 4737259 (1988-04-01), Ogawa et al.
patent: 4762743 (1988-08-01), von Alven et al.
patent: 4833332 (1989-05-01), Robertson, Jr. et al.
patent: 4834854 (1989-05-01), Sugihara et al.
patent: 4844786 (1989-07-01), Sugihara et al.
patent: 4897306 (1990-01-01), Sugimoto et al.
patent: 4904366 (1990-02-01), Tokita et al.
patent: 4954236 (1990-09-01), Kushner et al.
patent: 5084356 (1992-01-01), Deak et al.
patent: 5085904 (1992-02-01), Deak et al.
patent: 5190629 (1993-03-01), Sugihara et al.
patent: 5190632 (1993-03-01), Fujimiya et al.
patent: 5230781 (1993-07-01), Middendorf et al.
patent: 5498475 (1996-03-01), Takigawa et al.
patent: 5685967 (1997-11-01), Manis et al.
Vitor M.C. Madeira et al "Bargain Electrophoresis" Journal of Chemical Education, vol. 63, No. 12, pp. 1109-111, Dec. 1986.

Affiliated with

Also associated with

Rating

Gel cassette for electrophoresis does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Gel cassette for electrophoresis, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Gel cassette for electrophoresis will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-517532