GEF-H1b: biomarkers, complexes, assays and therapeutic uses...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S007200, C435S007210, C435S007230, C424S139100, C424S158100, C530S387100, C530S387900, C530S300000, C530S340000

Reexamination Certificate

active

07871786

ABSTRACT:
The present invention relates to diagnosing abnormal cell proliferation in biological samples and screening for drugs which inhibit, reduce or abolish cell growth, especially tumorigenic cell growth, by detecting a phosphovariant isoform of a guanine nucleotide exchange factor biomarker, such as the novel GEF-H1S.

REFERENCES:
patent: 5863532 (1999-01-01), Traugh et al.
patent: 2005/0100554 (2005-05-01), Jackson et al.
patent: 44 12 174 (1995-10-01), None
patent: WO 88/06598 (1988-09-01), None
patent: WO 99/53036 (1999-10-01), None
patent: WO 99/63073 (1999-12-01), None
patent: WO 01/32687 (2001-05-01), None
Callow et al., J. Biol. Chem., Jan. 2002, vol. 277(1):550-558.
Zenke et al., J. Biol. Chem., 2004, vol. 279(18):18392-18400.
Abo et al., “PAK4, a novel effector for Cdc42Hs, is implicated in the reorganization of the actin cytoskeleton and in the formation of filopodia,”The EMBO Journal, 1998, pp. 6527-6540, vol. 17, No. 22, Oxford University Press.
Callow et al., “Requirement for PAK4 in the Anchorage-independent Growth of Human Cancer Cell Lines,”The Journal of Biological Chemistry, Jan. 4, 2002, pp. 550-558, vol. 277, No. 1, The American Society for Biochemistry and Molecular Biology, Inc., USA.
Chan et al., “Expression cDNA cloning of a novel oncogene with sequence similarity to regulators of small GTP-binding proteins,”Oncogene, 1994, pp. 1057-1063, vol. 9, Macmillan Press Ltd.
Cravchik et al., “A novel strategy for the immunological tagging of cDNA constructs,”Gene, 1993, pp. 139-143, vol. 137, Elsevier Science Publishers B.V.
Dan et al., “Cytoskeletal Changes Regulated by the PAK4 Serine/Threonine Kinase Are Mediated by LIM Kinase 1 and Cofilin,”The Journal of Biological Chemistry, Aug. 24, 2001, pp. 32115-32121, vol. 276, No. 34, The American Society for Biochemistry and Molecular Biology, Inc., USA.
Dan et al., “PAK5, a New Brain-Specific Kinase, Promotes Neurite Outgrowth in N1E-115 Cells,”Molecular and Cellular Biology, Jan. 2002, pp. 567-577, vol. 22, No. 2, American Society for Microbiology.
Deo et al., “Green Fluorescent Protein Mutant as Label in Homogeneous Assays for Biomolecules,”Analytical Biochemistry, 2001, pp. 52-59, vol. 289, Academic Press.
Gauthier-Rouvière et al., “ras-induced c-fosexpression and proliferation in living rat fibroblasts involves C-kinase activation and the serum response element pathway,”The EMBO Journal, 1990, pp. 171-180, vol. 9, No. 1, Oxford University Press.
Gnesutta et al., “The Serine/Threonine Kinase PAK4 Prevents Caspase Activation and Protects Cells from Apoptosis,”The Journal of Biological Chemistry, Apr. 27, 2001, pp. 14414-14419, vol. 276, No. 17, The American Society for Biochemistry and Molecular Biology, Inc., USA.
Hashimura et al., “Production of Rabbit Antibody Specific for Amino-Terminal Residues of Cholecystokinin Octapeptide (CCK-8) by Selective Suppression of Cross-Reactive Antibody Response,”Journal of Immunological Methods,1982, pp. 375-387, vol. 55, Elsevier Biomedical Press.
Krendel et al., “Nucleotide exchange factor GEF-H1 mediates cross-talk between microtubules and the actin cytoskeleton,”Nature Cell Biology, Apr. 2002, pp. 294-301, vol. 4, No. 4.
Musacchio et al., “The PH domain: a common piece in the structural patchwork of signaling proteins,”Trends in Biochemical Sciences, Sep. 1993, pp. 343-348, vol. 18, The International Union of Biochemistry and Molecular Biology and Elsevier Trends Journals.
Nims et al., “Production of Hyperimmune Serum With Mature Rabbits,”Laboratory Animal Science, 1973, pp. 391-396, vol. 23, No. 3, American Association for Laboratory Animal Science, USA.
Nobes et al., “Rho, rac and cdc42 GTPases: regulators of actin structures, cell adhesion and motility,”Biochemical Society Transactions, 654thMeeting Leicester, Aug. 1995, pp. 456-459, vol. 23, No. 3.
O'Shea et al., “The ins and outs of cell-polarity decisions,”Nature Cell Biology, Mar. 2000, pp. E39-E41, vol. 2, No. 3.
Pandey et al., “Cloning and Characterization of PAK 5, a novel member of mammalian p21-activated kinase-II subfamily that is predominantly expressed in brain,”Oncogene, 2002, pp. 3939-3948, vol. 21, Nature Publishing Group.
Qu et al., “Activated PAK4 Regulates Cell Adhesion and Anchorage-Independent Growth,”Molecular and Cellular Biology, May 2001, pp. 3523-3533, vol. 21, No. 10, American Society for Microbiology.
Reddy et al., “Isolation and Characterization of Complementary DNA to Proliferating Cell Nucleolar Antigen P40,”Cancer Research, Apr. 1, 1989, pp. 1763-1767, vol. 49, No. 7, American Association for Cancer Research.
Ren et al., “Cloning and Characterization of GEF-H1, a Microtubule-associated Guanine Nucleotide Exchange Factor for Rac and Tho GTPases,”The Journal of Biological Chemistry, Dec. 25, 1998, pp. 34954-34960, vol. 273, No. 52, The American Society for Biochemistry and Molecular Biology, Inc.
Sato et al., “Fluorescent indicators for imaging protein phosphorylation in single living cells,”Nature Biotechnology, Mar. 2002, pp. 287-294, vol. 20, No. 3.
Shamah et al., “EphA Receptors Regulate Growth Cone Dynamics through the Novel Guanine Nucleotide Exchange Factor Ephexin,”Cell, Apr. 20, 2001, pp. 233-244, vol. 105, Cell Press.
Taylor et al., “Nonradioactive Determination of Ras-GTP levels Using Activated Ras Interaction Assay,”Methods in Enzymology, 2001, pp. 333-342, vol. 333, Academic Press.
Yang et al., “Androgen Receptor Specifically with a Novel p21-activated Kinase, PAK6,”The Journal of Biological Chemistry, May 4, 2001, pp. 15345-15353, vol. 276, No. 18, The American Society for Biochemistry and Molecular Biology, Inc., USA.
Zozulya et al., “Mapping signal transduction pathways by phage display,”Nature Biotechnology, Dec. 1999, pp. 1193-1198, vol. 17, Nature America Inc.
Ren et al., “Cloning and characterization of GEF-H1, a microtubule-associated guanine nucleotide exchange for Rac and Rho GTPases,”J. Biol. Chem., Dec. 25, 1998, pp. 34954-34960, vol. 273, No. 5.
Whitehead et al., “Expression cloning of lfc, a novel oncogene with structural similarities to guanine nucleotide exchange factors and to the regulatory region of protein kinase C,”J. Biol. Chem., Aug. 4, 1995, pp. 18388-18395, vol. 275, No. 31.
Lexikon der Biochemie und Molekularbiologie, 1991, pp. 65-69, Herder Verlag.
Scacco et al., J. Biol. Chem., 2000, vol. 275(23), pp. 17578-17582.
Krendel et al., Nature Cell Biol., 2002, 4, pp. 294-301.
Ren et al., J. Biol. Chem., 1998, vol. 273(52), pp. 34954-34960.
Callow et al., “PAK4 Mediates Morphological Changes Through the Regulation of GEF-H1,” Journal of Cell Science, vol. 118, No. Pt. 9, pp. 1861-1872 (2005).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

GEF-H1b: biomarkers, complexes, assays and therapeutic uses... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with GEF-H1b: biomarkers, complexes, assays and therapeutic uses..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and GEF-H1b: biomarkers, complexes, assays and therapeutic uses... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-2659614

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.