Gdp-4-keto-6-deoxy-d-mannose-3,5-epimerase-4-reductase gene...

Details Gdp-4-keto-6-deoxy-d-mannose-3,5-epimerase-4-reductase gene... Gdp-4-keto-6-deoxy-d-mannose-3,5-epimerase-4-reductase gene...

2007-04-03

2007-04-03

Rao, Manjunath (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Isomerase

C435S004000, C435S006120, C435S041000, C435S069100, C435S183000, C435S189000, C435S252300, C435S320100, C536S023200, C536S023740, C536S023400, C530S350000

Reexamination Certificate

active

10089014

ABSTRACT:
The present invention relates to a gene for an enzyme involving in the synthesis of GDP-L-fucose. Particularly, the present invention relates to a GDP-4-keto-6-deoxy-D-mannose-3, 5-epimerase-4-reductase gene derived fromArabidopsis thaliana, and a process for producing GDP-L-fucose using the gene. An enzyme encoded by the gene is (a) a protein comprising an amino acid sequence represented by SEQ ID NO: 1; or (b) a protein comprising an amino acid sequence derived from the amino acid sequence of SEQ ID NO: 1 by deletion, substitution, addition or insertion of one or several amino acid residues, and having GDP-4-keto-6-deoxy-D-mannose-3, 5-epimerase-4-reductase activity. The present invention enables efficient mass production of GDP-L-fucose which is essential in performing addition of fucose, which has a very important function in sugar chains.

REFERENCES:
patent: 0 870 841 (1998-10-01), None
patent: WO 99 36555 (1999-07-01), None
patent: WO 99/64618 (1999-12-01), None
Andrianopoulos et al. 1998 (J. Bacteriol., vol. 180(4):998-1001).
Bonin et al. 1997 (Plant Physiol., vol. 114(3), Sup. p. 22, Abstract 20.
SPTREMBL database accession No. O49213, Jun. 1998, Bonin et al.
Chang et al. J. Biol. Chem., 1988, vol. 263(4):1693-97.
M. Tonetti et al, “Preliminary crystallographic investigations of recombinant GDP-4-keto-6-deoxy-d-mannose epimerase/reductase fromE coli”, International Union of Crystallography; vol. 54, No. 4, pp. 684-686 (1996).
Abstract—Bonin et al., (1998) “Arabidopsis thaliana GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase (GERI) gene, complete cds.” Database accession No. AF045286 XP002176506.
Bonin et al. (1997) “The MURI gene of Arabidopsis thaliana encodes an isoform of GDP -D-mannose-4,6-dehydratase, catalyzing the first step in the de novo synthesis of GDP-L-fucose” Proc. Natl. Acad. Sci . USA 94(5):2085-2090.

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