Drug – bio-affecting and body treating compositions – Radionuclide or intended radionuclide containing; adjuvant... – Molecular bilayer structure
Patent
1976-04-12
1978-08-15
Meyers, Albert T.
Drug, bio-affecting and body treating compositions
Radionuclide or intended radionuclide containing; adjuvant...
Molecular bilayer structure
23230B, 195 63, 1951035A, 252408, 424 3, 424 11, 424 12, 424 13, G01N 3100, G01N 3102, G01N 3122, G01N 3316
Patent
active
041072871
ABSTRACT:
An immunochemical indicator reagent capable of indicating the presence of specific antigenic or haptenic material by hemagglutination reaction therewith, comprising the corresponding antibody globulin molecules chemically coupled to erythrocyte carrier particles through a bifunctional chlorine-terminated coupling agent consisting of either diazo blue B or diazo blue B-terminated condensation products of diazo blue B with glycine. The reagent is prepared by first treating the erythrocyte carrier particles with a proteolytic enzyme such as trypsin to provide the erythrocyte surface with additional exposed active hydrogen-containing groups including free amino groups, then fixing the erythrocyte carrier particles with a fixing agent, such as an aldehyde, and thereafter chemically coupling the erythrocyte carrier particles to the antibody globulin molecules by reaction with the bifunctional chlorine-terminated coupling agent. The coupling action of this coupling agent is preferentially through the free amino groups in each of the two materials being coupled, providing a chemical bond of high strength and stability, and further does not impair the antibody activity, resulting in the reagent having a high degree of sensitivity and stability. The indicator reagent may be used in performing hemagglutination tests directly with materials removed from animal bodies or with colonies of microorganisms or with tissue cells growing upon solid surfaces.
REFERENCES:
patent: 3930951 (1976-01-01), Messing
patent: 3983000 (1976-09-01), Messing
Prager, Fed. Proc., vol. 25, No. 2 P & I, Mar.-Apr. 1966, Ab. No. 337.
Borek, Nature, vol. 191, Sep. 1961, pp. 1293-1294.
Lam, Applied Microbiol., vol. 27, Feb. 1974, pp. 356-359.
Arrameas, Immunochemistry, vol. 6, 1969, pp. 67-76.
Lam Gow T.
Morton Harry E.
Fagelson A. P.
Meyers Albert T.
University of Pennsylvania
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