Gas permeable culture flask and method for culturing mammalian c

Details Gas permeable culture flask and method for culturing mammalian c Gas permeable culture flask and method for culturing mammalian c

1987-08-17

1991-09-10

Kamen, Noah P.

Chemistry: molecular biology and microbiology

Apparatus

Bioreactor

435284, 220371, C12M 124

Patent

active

050473476

ABSTRACT:
A gas permeable culture flask and method for culturing mammalian cells wherein a gas permeable insert is provided in the flask to allow rapid and uniform equilibration of gases between the atmosphere of the culture flask and the atmosphere of the incubator into which the flask is placed for culturing. The size of the gas permeable insert relative to the overall surface area of the flask is limited to allow the flask to be removed from the incubator for observation, testing and culture treatment for relatively long times without substantial changes to the flask atmosphere. Alternatively, removable covers are provided for occluding passage of gases through the gas permeable insert when the flask is removed from the controlled atmosphere of the incubator.

REFERENCES:
patent: 2950833 (1960-08-01), Short
patent: 3158553 (1964-11-01), Carski
patent: 4377247 (1983-03-01), Hazard et al.

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