Gas driven system for preparing large volumes of non-oxidized, p

Details Gas driven system for preparing large volumes of non-oxidized, p Gas driven system for preparing large volumes of non-oxidized, p

1986-10-10

1988-03-15

Jenkins, Robert W.

Agitating

By injecting gas into mixing chamber

261DIG28, 366137, 422 44, B01F 1302

Patent

active

047309369

ABSTRACT:
An apparatus and method for reacting dilute concentrations of delicate bioorganics or other reactants without creating microconcentrations of reactant during the reaction process. The apparatus mixes reagents inside of Y-connector with flowing solution from a solution reservoir. The reagents are introduced through a stopcock controlled infusion port at the center of the Y-connector. Solution and pressurized gas are each supplied to one of the three legs of the Y-connector and the resulting mixed solution from the third leg of the Y-connector is lifted by bubbles of the pressurized gas to return to the solution reservoir. A gaseous separation unit is placed inside the solution reservoir wherein the bubble lifted and mixed solution is forced through a permeable membrane enclosed by a perforated bulb to return to the reservoir. The perforated bulb is enclosed inside an additional enclosure having an opening below the solution level of the reservoir so that the returning solution will flow beneath the opening to the larger reservoir area and the excess gas will escape above the solution level through vent holes in the additional enclosure. Additional pressurized gas is delivered to the enclosure opening to bubble over the outside of the perforated bulb. A second Y-connector may be added between the first Y-connector and the solution reservoir.

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R. E. Benesch et al., "Affinity Labeling of the Polyphosphate Binding Site of Hemoglobin", Biochemistry, vol. 11, No. 19, Nov. 1972, pp. 3576-3582.
R. P. Ten Eyck et al., "Stroma-Free Methemoglobin Solution: an Effective Antidote for Acute Cyanide Poisoning", American Journal of Emergency Medicine, vol. 3, No. 6, Nov. 1985, pp. 519-523.

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