Chemistry: analytical and immunological testing – Peptide – protein or amino acid
Patent
1994-05-04
1996-09-24
Warden, Jill
Chemistry: analytical and immunological testing
Peptide, protein or amino acid
436120, 436161, 436173, 436174, 436178, G01N 3000
Patent
active
055590381
ABSTRACT:
Method for quantifying the amounts of oxidized sulfhydryl amino acids, particularly cysteine sulfinic acid, cysteic acid, homocysteine sulfinic acid and homocysteic acid, for example, in biological samples are provided. The oxidized sulfhydryl amino acids are measured by gas chromatography/mass spectrometry after derivatization, preferably using stable isotope internal standards. Deviation from normal individuals' levels of oxidized sulfhydryl amino acids in serum are indicative of certain neuropsychiatric disorders, the biochemical origin of which can be diagnosed using methods disclosed herein, particularly when relative levels of these and/or other sulfur amino acids are measured.
REFERENCES:
patent: 4940658 (1990-07-01), Allen et al.
Heafield et al. (1990) "Plasma Cysteine and Sulphate Levels in Patients with Motor Neurone, Parkinson's and Alzheimer's Disease", NeuraSci. Lett. 110:216-220.
Perry et al. (1991) "Amyotrophic Lateral Sclerosis: Fasting Plasma Levels of Cysteine and Inorganic Sulfate are Normal, as are Brain Contents of Cysteine", Neurology 41:487-490.
Cohen et al. (1974) "The Administration of Methionine to Chronic Schizophrenic Patients: A Review of Ten Studies", Biol. Psychiatry 8:209-225.
Deutsch et al. (1989) "A `Glutamatergic Hypothesis` of Schizophrenia Rationale for Pharmacotherapy with Glycine", Clin. Neuropharmacology 1(vol.12):1-13.
Olney, J. W. (1989) "Excitatory Amino Acids and Neuropsychiatric Disorders", Biol. Psychiatry 26:505-525.
Sharvon et al. (1980) "The Neuropsychiatry of Megaloblastic Anaemia", Br. Med. J. 281:1036-1038.
MacKenzie et al., J. Chromatogr. vol. 187 No. 1, pp. 239-243 (1980).
Kataoka et al., J. Chromatogr. vol. 354 pp. 482-485 (1986).
Ng et al., J. Chromatogr. vol. 513 pp. 61-69 (1990).
Santnosh-Kumar et al., Anal. Biochem, vol. 220 No. 2 pp. 249-256 (1994).
Grieve and Griffiths (1992) "Simultaneous Measurement by HPLC of the Excitatory Amino Acid Transmitter Candidates Homocysteate and Homocysteine Sulphinate Supports a Predominant Astrocytic Localisation", Neuroscience Letters 145:1-5.
Klancnik et al. (1992) "Release of Endogenous Amino Acids, Including Homocysteic Acid and Cysteine Sulphinic Acid, from Rat Hippocampal Slices Evoked by Electrical Stimulation of Schaffer Collateral-Commissural Fibres", Neuroscience 49:557-570.
Vahora et al. (1988) "S-Adenosylmethionine in the Treatment of Depression", Neurosci. & Behavioural Rev, 12:139-141.
Luchi and De Marco (1972) "Synthesis of Homocysteinesulfinic Acid", Anal. Biochem 45:236-241.
Lipton et al. (1994) "Excitatory Amino Acids as a Final Common Pathway for Neurologic Disorders", N.E.J. Med. 330:613-622.
Francis et al. (1989) "Brain Amino Acid Concentrations and Ca.sup.2+ -dependent Release in Intractable Depression Assessed Antemortem", Brain Res. 494:315-324.
Ohmori et al. (1972) "Unusual Sulfur-Containing Amino Acids in the Urine of Homocystinuric Patients: III. Homocysteic Acid, Homocysteine Sulfinic Acid, S-(Carboxymethylthio) Homocysteine, and S-(3-Hydroxy-3-Carboxy-n-Propyl) Homocysteine", Physiol. Chem. & Physics. 4:286-294.
Perry et al. (1985) "Hallervorden-Spatz Disease: Cysteine Accumulation and Cystein Dioxygenase Deficiency in the Globus Pallidus", Ann. Neurol. 18:482-489.
Misra and Olney (1975) "Cysteine Oxidase in Brain", Brain Res. 97:117-126.
Mewett et al. (1983) "Pharmacology of the Excitatory Actions of Sulphonic and Sulphinic Amino Acids", in Mandell, P., DeFeudis, F. V. (eds) CNS Receptors: From Pharmacology to Behaviour, 163-174, Raven Press, New York.
Deusch, J. C. and Kolhouse, J. F. (1993) "Ascorbate and Dehydroascorbate Measurements in Aqueous Solutions and Plasma Determined by Gas Chromatography--Mass Spectrometry", Anal. Chem. 65:321-326.
Do et al. (1986) "In Vitro Release of Endogenous Excitatory Sulfur-Containing Amino Acids from Various Rat Brain Regions", J. Neurochem. 46:779-786.
Griffiths, R. (1993) "The Biochemistry and Pharmacology of Excitatory Sulphur-Containing Amino Acids", Biochem. Soc. Trans. 21:66-72.
Mudd et al. (1989) "The Metabolic Basis of Inherited Disease", in Scriver, C. R. et al. (eds) Disorders of Transsulfuration, pp. 693-734, New York, McGraw Hill.
Cox et al. (1977) "Actions of L-and D-Homocysteate in Rat CNS: A Correlation between Low-Affinity Uptake and the Time Courses of Excitation by Microelectrophoretically Applied L-Glutamate Analogues", J. Neurochem 29:579-588.
Deutsch John C.
Kolhouse J. Fred
Santhosh-Kumar Cheruppolil R.
Ludlow Jan M.
The Regents of the University of Colorado
Warden Jill
LandOfFree
Gas chromatography/mass spectrometry determination of oxidized s does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Gas chromatography/mass spectrometry determination of oxidized s, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Gas chromatography/mass spectrometry determination of oxidized s will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1928163