.gamma.-phosphate linked adenosine 5' triphosphate sepharose

Details .gamma.-phosphate linked adenosine 5' triphosphate sepharose .gamma.-phosphate linked adenosine 5' triphosphate sepharose

1994-03-09

1996-07-16

Rollins, John W.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

435194, 435815, 210690, 210691, 210692, C07H 1920, C12N 912, B01D 1508

Patent

active

055368220

ABSTRACT:
A .gamma.-phosphate-linked ATP affinity column for the purification of a protein kinase is provided. In addition, a method for purifying protein kinases using the .gamma.-phosphate-linked ATP affinity column comprising the steps of 1) linking an ATP linking moiety to an affinity column via the .gamma.-phosphate position of ATP; 2) applying a sample of protein kinase to the .gamma.-phosphate linked ATP solid support so as to bind the protein kinase to the .gamma.-phosphate-linked ATP; 3) washing the bound support with a salt which will not affect the binding of the bound protein kinase; and 4) eluting the bound protein kinase with an ATP salt so as to obtain homogenous protein kinase is provided.

REFERENCES:
Haystead et al, Eur. J. Biochem. 214 (2): 459-467 (1993).
Berglund et al, Eur. J. Biochem. 28: 492-496 (1972).
"Affinity Chromatography, Principles and Methods" Published by Pharmacia Fine Chemicals (1974).

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