.gamma.-chain humaninterleukin-2 receptor polypeptide and method

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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435 691, 4352523, 4353201, 435239, 536 2353, 536 235, 53038822, C12P 2104, C12P 2106, C12N 120, C12N 1500

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058561404

ABSTRACT:
A polypeptide which specifically binds to the .gamma.-chain of the human interleukin-2 receptor and selectively inhibits the binding of the .gamma.-chain of human interleukin-2 receptor to the .beta.-chain of the same is provided. The polypeptide has an activity of blocking the human interleukin-2 response. Also provided are an immunosuppressant containing the polypeptide, a DNA gene coding for the polypeptide, a recombinant DNA having the gene, a transformant having the recombinant DNA, and a method for producing the intended polypeptide by incubating the transformant. The novel polypeptide can be used independently, or with substances capable of inhibiting the binding of interleukin-2 to the interleukin-2 receptor, as a medicine for preventing the rejection of grafts after transplantation and also for curing inflammatory diseases such as allergic diseases and autoimmune diseases.

REFERENCES:
Bird, R.E. et al, Science, 242:423-426, Oct. 21, 1988.
Tanalsa, Y. et al, Microbiol Immun, 29(10):959-972, 1985.
Talseshita, T. et al, Science, 257:379-382, Jul. 17, 1992.
S.D. Voss et al, "Identification of a direct interaction between interleukin 2 and th p. 64 interleukin 2 receptor .gamma. chain", March 1993, Proc. Natl. Acad. Sci. USA, vol. 90., pp. 2428-2432.
M. Kondo et al, "Sharing of the interleukin-2 (IL-2) receptor .gamma. chain between receptors for IL-2 and IL-4", Dec. 17, 1973, Science, vol. 262., pp. 1874-1877.
M. Fujii et al, "High-and low-affinity interleukin 2 receptors: Distinctive effects of monoclonal antibodies", Sep. , 1986, The Journal of Immunology, vol. 137., No. 5., pp. 1552-1556.

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