Galactomannan-agarose binary gel for nucleic acid electrophoresi

Details Galactomannan-agarose binary gel for nucleic acid electrophoresi Galactomannan-agarose binary gel for nucleic acid electrophoresi

1991-01-24

1993-07-27

Stoll, Robert L.

Compositions

Preservative agents

Anti-corrosion

2523154, 2041828, 204299R, 522911, 521 505, B01J 1300, C25B 700, B01D 6142, G01N 2726

Patent

active

052308324

ABSTRACT:
A method for forming an agarose electrophoretic gel by addition of gamma-irradiated clarified galactomannan (.gamma.CGM) to optically clarify the gel and improve the electrophoretic fractionation and resolution of oligonucleotides differing in molecular weight. The gel includes agarose, an electrophoretic buffer for nucleic acids, and a CGM vegetable gum subjected to between 0.1 and 4.0 megarads of ionizing gamma radiation.

REFERENCES:
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patent: 4290911 (1981-09-01), Cook et al.
patent: 4321121 (1982-03-01), Gurske
patent: 4704198 (1987-11-01), Ebersole et al.
patent: 5114550 (1992-05-01), Schomberg et al.
Chemical Abstracts, CA 70(15): 65022y.
Perlman et al., Analytical Biochemistry, 163, 247-254 (1987).
Peacock et al., Biochemistry, 7(2), 668-674 (1968).

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