Gabaa receptor epsilon subunits

Details Gabaa receptor epsilon subunits Gabaa receptor epsilon subunits

2006-04-18

2006-04-18

Landsman, Robert E. (Department: 1647)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C435S252300, C435S320100, C435S325000, C435S471000, C536S023500

Reexamination Certificate

active

07029870

ABSTRACT:
The present invention relates to a novel GABAAreceptor ε subunit (GABRE) and an alternative transcript thereof (ET2). More specifically, isolated nucleic acid molecules are provided encoding human GABRE and ET2 receptor subunits. ET2 and GABRE polypeptides are also provided, as are vectors, host cells and recombinant methods for producing the same. The invention further relates to screening methods for identifying agonists and antagonists of ET2 and GABRE activities. Also provided are diagnostic methods for detecting aberrant GABRE and ET2 expression, as well as therapeutic methods for treating disorders involving ET2 and GABRE.

REFERENCES:
patent: WO 94/13799 (1884-06-01), None
patent: WO 96/06862 (1996-03-01), None
patent: WO 98/23742 (1998-06-01), None
Davies et al. Insensitivity to anaesthetic agents conferred by a class of GABA-a receptor subunit. Nature. vol. 385, pp. 820-823, 1997.
Garret et al. An nRNA encoding a putative GABA-gated chloride channel is expressed in the human cardiac conduction system. J. Neurochem. vol. 68, pp. 1382-1389, 1997.
Reichmann et al. Activation of an inducible c-fosER fusion protein causes loss of epithelial polarity and triggest epithelial-fibroblastoid cell conversion. Cell. vol. 71, pp. 1103-1116, 1992.
Adams, M.D. et al., “Initial assessment of human gene diversity and expression patterns based upon 83 million nucleotides of cDNA sequence,”Nature 377(Supp.):3-174 (1995).
Adodra, S. and Hales, T.G., “Potentiation, activation and blockade of GABAAreceptors of clonal murine hypothalamic GT1-7 neurones by propofol,”Br. J. Pharmacol. 115(6):953-960 (1995).
Amann, E. et al., “Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins inEscherichia coli,” Gene 69(2):301-315 (1988).
Bateson, A.N. et al., “γ-Aminobutyric AcidAReceptor Heterogeneity Is Increased by Alternative Splicing of a Novel β-Subunit Gene Transcript,”J. Neurochem. 56(4):1437-1440 (1991).
Bell, K. et al., “GABAergic Projection From the Ventral, Pallidum and Globus Pallidus to the Subthalamic Nucleus,”Synapse 20(1):10-18 (1995).
Bergman, H. et al., “Reversal of Experimental Parkinsonism by Lesions of the Subthalamic Nucleus,”Science 249:1436-1438 (1990).
Bormann, J. et al., “Mechanism of Anion Permeation through Channels Gated by Glycine and γ-Aminobutyric Acid in Mouse Cultured Spinal Neurones,”J. Physiol.(London)385:243-286 (1987).
Buckle, V.J. et al., “Chromosomal Localization of GABAAReceptor Subunit Genes: Relationship to Human Genetic Disease,”Neuron 3:647-654 (1989).
Buller, A.L. et al., “Site-Directed Mutagenesis of N-Linked Glycosylation Sites on the γ-Aminobutyric Acid Type A Receptor α1 Subunit,”Mol. Pharmacol. 46(5):858-865 (1994).
Davies, P.A. et al., “Insensitivity to anaesthetic agents conferred by a class of GABAAreceptor subunit,”Nature 385:820-823 (Feb. 1997).
Davies, P.A. et al., “Modulation by general anaesthetics of rat GABAAreceptors comprised of α1β3 and β3 subunits expressed in human embryonic kidney 293 cells,”Br. J. Pharmacol. 120(5):899-909 (Mar. 1997).
Gage, P.W. and Chung, S.-H, “Influence of membrane potential on conductance sublevels of chloride channels activated by GABA,”Proc. R. Soc. Lond. B 255(1343):167-172 (1994).
Garret, M. et al., “An mRNA Encoding a Putative GABA-Gated Chloride Channel Is Expressed in the Human Cardiac Conduction System,”J. Neurochem. 68:1382-1389 (Apr. 1997).
Goetz, C.G. and Diederich, N.J., “There is a renaissance of interest in pallidotomy for Parkinson's disease,”Nature Med. 2(5):510-514 (May 1996).
Gray, R. and Johnston, D., “Rectification of Single GABA-Gated Chloride Channels in Adult Hippocampal Neurons,”J. Neurophysiol. 54(1):134-142 (1985).
Greger, V. et al., “The γ-Aminobutyric Acid Receptor γ3 Subunit Gene (GABRG3) Is Tightly Linked to the α5 Subunit Gene (GABRA5) on Human Chromosome 15q11-q13 and is Transcribed in the Same Orientation,”Genomics 26:258-264 (1995).
Hedblom, E. and Kirkness, E.F., “A Novel Class of GABAAReceptor Subunit in Tissues of the Reproductive System,”J. Biol. Chem. 272(24):15346-15350 (Jun. 1997).
Hicks, A.A. et al., “Further Evidence for Clustering of Human GABAAReceptor Subunit Genes: Localization of the α6-Subunit Gene (GABRA6) to Distal Chromosome 5q by Linkage Analysis,”Genomics 20:285-288 (1994).
Kirkness, E.F. et al., “Isolation, Characterization, and Localization of Human Genomic DNA Encoding the β1 Subunit of the GABAAReceptor (GABRB1),”Genomics 10:985-995 (1991).
Knoll, J.H.M. et al., “Fish ordering of reference markers and of the gene for the α5 subunit of the γ-aminobutyric acid receptor (GABRA5) within the Angelman and Prader-Willi syndrome chromosomal regions,”Human Molecular Genetics 2(2):183-189 (1993).
Korpi, E.R. and Lüddens, H., “Regional γ-Ambinobutyric Acid Sensitivity of t-Butylbicyclophosphoro[35S]thionate Binding Depends on γ-Aminobutyric AcidAReceptor α Subunit,”Mol. Pharmacol. 44(1):87-92 (1993).
Lasham, A. et al., “Conserved Organization of γ-Aminobutyric AcidAReceptor Genes: Cloning and Analysis of the Chicken β4-Subunit Gene,”J. Neurochem. 57(1):352-355 (1991).
Lewohl, J.M. et al., “Expression of the α1, α2and α3isoforms of the GABAAreceptor in human alcoholic brain,”Brain Research 751:102-112 (Mar. 1997).
Macdonald, R.L. and Olsen, R.W., “GABAAReceptor Channels,”Annu. Rev. Neurosci. 17:569-602 (1994).
Matsuyama, S. et al., “GABA modulates neurostransmission in sinus node via stimulation of GABAAreceptor,”Am. J. Physiol. 264(4):H1057-H1061 (1993).
McLean, P.J. et al., “Mapping of the α4Subunit Gene (GABRA4) to Human Chromosome 4 Defines an α2-α4-β1-γ1Gene Cluster: Further Evidence That Modern GABAAReceptor Gene Clusters Are Derived from an Ancestral Cluster,”Genomics 26:580-586 (1995).
McLemore, G.L. et al., “Cardiac Noradrenergic Mechanisms Mediate GABA-Enhanced Ouabain Cardiotoxicity,”Pharmacology 49:343-350 (1994).
Peters, J.A. et al., “Modulation of the GABAAreceptor by depressant barbiturates and pregnane steroids,”Br. J. Pharmacol. 94(4):1257-1269 (1988).
Rogner, U.C. et al., “A YAC clone map spanning 7.5 megabases of human chromosome band Xq28,”Human Molecular Genetics 3(12):2137-2146 (1994).
Russek, S.J. and Farb, D.H., “Mapping of the β2Subunit Gene (GABRB2) to Microdissected Human Chromosome 5q34-q35 Defines a Gene Cluster for the Most Abundant GABAAReceptor Isoform,”Genomics 23:528-533 (1994).
Sanna, E. et al., “Actions of the General Anesthetic Propofol on Recombinant GABAAReceptors: Influence of Receptor Subunits,”J. Pharmacol. Exp. Ther. 274(1):353-360 (1995).
Sargent, P.B., “The Diversity of Neuronal Nicotinic Acetylcholine Receptors,”Annu. Rev. Neurosci. 16:403-443 (1993).
Saxena, N.C. and Macdonald, R.L., “Assembly of GABAAReceptor Subunits: Role of the δ Subunit,”J. Neurosci. 14(11):7077-7086 (1994).
Schmieden, V. et al., “Agonist pharmacology of neonatal and adult glycine receptor α subunits: identification of amino acid residues involved in taurine activation,”EMBO J. 11(6):2025-2032 (1992).
Segal, M. and Barker, J.L., “Rat Hippocampal Neurons in Culture: Properties of GABA-Activated CL-Ion Condutance,”J. Neurophysiol. 51(3):500-515 (1984).
Simmons, D. and Seed, B., “Isolation of a cDNA Encoding CD33, a Differentiation Antigen of Myeloid Progenitor Cells,”J. Immunol. 141(8):2797-2800 (1988).
Smith, T.F. and Waterman, M.S., “Comparison of Biosequences,”Adv. Appl. Math. 2:482-489 (1981).
Sommer, B. et a

Affiliated with

Also associated with

Rating

Gabaa receptor epsilon subunits does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Gabaa receptor epsilon subunits, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Gabaa receptor epsilon subunits will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3587585