GA4 DNA, protein and method of use

Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part

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800278, 800286, 800290, 536 236, 536 241, 536 245, 435 691, 435 701, 4352523, 4352542, 4353201, 435419, 435468, A01H 500, A01H 510, C12N 504, C12N 1529

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059258071

ABSTRACT:
The invention relates to the DNA and protein encoded by the GA4 locus. This protein is believed to be a member of the family of enzymes involved in the biosynthesis of the gibberellin family (GA) of plant growth hormones which promote various growth and developmental processes in higher plants, such as seed germination, stem elongation, flowering and fruiting. More specifically, the protein encoded by the GA4 locus is an hydroxylase. The invention also relates to vectors containing the DNA and the expression of the protein encoded by the DNA of the invention in a host cell. Additional aspects of the invention are drawn to host cells transformed with the DNA or antisense sequence of the invention, the use of such host cells for the maintenance, or expression or inhibition of expression of the DNA of the invention and to transgenic plants containing DNA of the invention. Finally, the invention also relates to the use of the protein encoded by the GA4 locus to alter aspects of plant growth.

REFERENCES:
patent: 5110728 (1992-05-01), Kridl et al.
Berry-Lowe et al., "The Nucleotide Sequence, Expression, and Evolution of One Member of a Multigene Family Encoding the Small Subunit of Ribulose-1,5-Bisphosphate Carboxylase in Soybean," J. Mol. Appl. Genet. 1(6):483-498 (1982).
Bevan, M., "Binary Agrobacterium vectors for plant transformation," Nucl. Acids Res. 12(22):8711-8721 (1984).
Britsch et al., "Molecular Cloning, Sequence Analysis, and in vitro Expression of Flavanone 3.beta.-Hydroxylase from Petunia hybrida," J. Biol. Chem. 267(8):5380-5387 (1992).
Cashmore, A.R., "Nuclear Genes Encoding the Small Subunit of Ribulose-1,5-Bisphosphate Carboxylase," in: Genetic Engineering of Plants, An Agricultural Perspective, Plenium Press, New York, pp. 29-38 (1983).
Coruzzi et al., "Nucleotide Sequences of Two Pea cDNA Clones Encoding the Small Subunit of Ribulose 1,5-Bisphosphate Carboxylase and the Major Chlorophyll a/b-binding Thylakoid Polypeptide," J. Biol. Chem. 258(3):1399-1402 (1983).
Deikman and Fischer, "Interaction of a DNA binding factor with the 5'-flanking region of an ethylene-responsive fruit ripening gene from tomato," EMBO J. 7(11):3315-3320 (1988).
Dunsmuir et al., "The Major Chlorophyll a/b Binding Protein of Petunia Is Composed of Several Polypeptides Encoded by a Number of Distinct Nuclear Genes," J. Mol. Appl. Genet. 2(3):285-300 (1983).
Feldmann and Marks, "Agrobacterium-mediated transformation of germinating seeds of Arabidopsis thaliana: A non-tissue culture approach," Mol. Gen. Genet. 208:1-9 (1987).
Fraley et al., "Expression of bacterial genes in plant cells," PNAS USA 80:4803-4807 (1983).
Fujioka et al., "Qualitative and Quantitative Analyses of Gibberellins in Vegetative Shoots of Normal, dwarf-1, dwarf-2, dwarf-3, and dwarf-5 Seedlings of Zea Mays L.," Plant Physiol. 88:1367-1372 (1988).
Horsch et al., "Inheritance of Functional Foreign Genes in Plants," Science 233:496-498 (1984).
Ingram et al., "Internode length in Pisum," Planta 160:455-463 (1984).
Koornneef and van der Veen, "Induction and Analysis of Gibberellin Sensitive Mutants in Arabidopsis thaliana (L.) Heynh.," Theor. Appl. Genet. 58:257-263 (1980).
Meldgaard, M., "Expression of chalcone synthase, dihydroflavonal reductase, and flavanone-3-hydroxylase in mutants of barley deficient in anthocyanin and proanthocyanidin biosynthesis," Theor. Appl. Genet. 83:695-706 (1992).
Moritz and Monteiro, "Analysis of endogenous gibberellins and gibberellin metabolites from Dalbergia dolichopetala by gas chromatography-mass spectrometry and high-performance liquid chromatography-mass spectrometry," Planta 193:1-8 (1994).
Odell et al., "Identification of DNA Sequences required for activity of the cauliflower mosaic virus 35S promoter," Nature 313:810-812 (1985).
Phinney and Spray, "Chemical Genetics and the Gibberellin Pathway in Zea mays L.," In: Plant Growth Substances, P.F. Waering, Ed., Academic Press, NY, pp. 101-110 (1982).
Ross et al., "Internode length in Pisum. Estimation of GA.sub.1 levels in genotypes Le, le and le.sup.d," Physiol. Plant. 76:173-176 (1989).
Talon et al., "Endogenous gibberellins in Arabidopsis thaliana and possible steps blocked in the biosynthetic pathways of the semidwarf ga4 and ga5 mutants," PNAS USA 87:7983-7987 (1990).
van der Krol et al., "Antisense genes in plants: an overview," Gene 72:45-50 (1988).
van der Krol et al., "Inhibition of flower pigmentation by antisense CHS genes: promoter and minimal sequence requirements for the antisense effect," Plant Mol. Biol. 14:457-466 (1990).
Walden et al., "T-DNA as a gene tag," Plant J. 1(3):281-288 (1991).
Zhang et al., "Expression of Antisense or Sense RNA of an Ankyrin Repeat-Containing Gene Blocks Chloroplast Differentiation in Arabidopsis," Plant Cell 4:1575-1588 (1992).
Britsch et al., "Molecular Characterization of Flavonone 3Beta-hydroxylases, Consesus Sequence, Comparison with Related Enzymes and the Role of Conserved Histidine Residues," Eur. J. Biochem. 217:745-754 (Oct. 1993).
Chiang et al., "Isolation of the Arabidopsis GA4 Locus," The Plant Cell 7(2): 195-201 (1995).
Dehio et al., "Phenotype and Hormonal Status of Transgenic Tobacco Plants Overexpressing the rolA Gene of Agrobacterium rhizogenes T-DNA," Plant Molecular Biology 23: 1199-1210 (Dec. 1993).
Lange et al., "Separation and Characterisation of Three 2-Oxoglutarate-dependent Dioxygenases from Curcurbita maxima L. Endosperm Involved in Gibberellin Biosynthesis," Planta 195(1): 98-107 (Apr. 1994).
Nilsson et al., "Hormonal Characterization of Transgenic Tobacco Plants Expressing the rolC Gene of Agrobacterium rhizogenes TL-DNA," Plant Physiol. 102: 363-371 (Jun. 1993).
Schmulling et al., "Hormonal Content and Sensitivity of Transgenic Tobacco and Potato Plants Expressing Single ro1 Genes of Agrobacterium rhizogenes T-DNA," The Plant Journal 3(3): 371-382 (Mar. 1993).
Smith et al., "Partial Purification and Characterization of the Gibberellin A20 3Beta-hydroxylase from seeds of Phaseolus vulgaris," Plant Physiol. 94(3): 1390-1401 (1990).
Spray et al., "Internode Length in Zea mays (L). The Dwarf-1 Mutation Controls the 3 Beta-hydroxylation of Gibberellin A20 to Gibberellin A1," Planta 160: 464-468 (1984).
Smith et al. 1988. Nature 334:724-726.
Napoli et al. 1990. The Plant Cell 2:279-289.

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