Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2007-11-06
2007-11-06
Ulm, John (Department: 1649)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C435S252300, C435S320100, C536S023400
Reexamination Certificate
active
10952773
ABSTRACT:
The invention provides a series of Gαqprotein variants that functionally couple to sensory cell receptors such as taste GPCRs (TRs) and olfactory GPCRs (ORs) in an overly promiscuous manner. According to the invention, the functional coupling can be determined, for example, by measuring changes in intracellular IP3, or calcium. In a particular embodiment, the Gαqprotein variants can be expressed in mammalian cell lines orXenopusoocytes, and then evaluated using calcium fluorescence imaging and electrophysiological recording.
REFERENCES:
Mody et al. Incorporation of G alpha z-Specific Sequence at the Carboxyl Terminus Increases the Promiscuity of Galpha16 toward Gi-coupled Receptors. Jan. 2000, Molecular Pharmacology 57:13-23.
Kostenis et al. Functional Characterization of a Series of Mutant G Protein alpha q Subunits Displaying Promiscuous Receptopr Coupling Properties. J. Biol. Chem. 273(28):17886-17892.
Strathmann et al. G Protein Diversity: A Distinct Class of alpha Subunits isPresent in Vertibrates and Invertebrates. P.N.A.S. 87:9113-9117.
Xu Hong
Yao Yong
Hunton & Williams LLP
Senomyx Inc.
Ulm John
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