Fusobacterium nucleic acids, plasmids and vectors

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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C536S023100, C435S320100, C435S476000, C435S488000, C435S252300, C435S252330, C530S350000

Reexamination Certificate

active

06962990

ABSTRACT:
The invention provides origin of replication sequences and replication genes and proteins for a plasmid functional inFusobacterium(e.g.,F. nucleatum) and related species. Provided by the invention are also plasmids and vectors that can replicate inFusobacterium. Further, the invention provides shuttle vectors that can replicate inFusobacteriumand in other microorganisms, such asE. coli. Still further, the present invention provides host cells comprising the plasmids and shuttle vectors, and methods for transformation of the host cells with the plasmid and shuttle vectors of the invention.

REFERENCES:
GenBank Accession No. AF022647, established on Sep. 3, 1997.
Chang et al. The Journal of Cell Biology 104: 1563-1568, 1987.
Kelley et al. Endocrine Research 16:477-491, 1990.
Everett et al. Pendred syndrome is caused by mutations in a putative sulphate transporter gene (PDS). Nature Genetics 17: 411-422, 1997.
Scott et al. The pendred syndrome gene encodes a chloride-iodide trasnport protein. Nature Genetics 21: 440-443, 1999.
Kinder Haake et al. Journal of Dental Research 78(abstracts):420; Abstract No. 2498, 1999.
Kinder Haake et al. Abstracts of the General Meeting of the American Society of Microbiology 99:331, Abstract No. H-9, May 1999.
McKay et al. Plasmid 33:15-25 1995.
Sptrembl database result of SEQ ID NO:1 (RepA protein) against RepA sequence disclosed by McKay et al.

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