Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Bacterium or component thereof or substance produced by said...
Patent
1993-06-18
1995-10-03
Lilling, Herbert J.
Drug, bio-affecting and body treating compositions
Antigen, epitope, or other immunospecific immunoeffector
Bacterium or component thereof or substance produced by said...
4241301, 4241641, 4241761, 4241841, 42419711, 435 713, 435243, 4352521, 435822, A61K 3900, A61K 3902
Patent
active
054550343
ABSTRACT:
A method is provided for the enhanced elaboration of leukotoxin from F. necrophorum, and subsequent production of an inactivated leukotoxoid ruminant animal vaccine against F. necrophorum infection and consequent liver abscesses and/or foot rot in such animals. The method involves forming a culture of F. necrophorum bacteria in growth media, allowing the bacteria to grow therein and to simultaneously elaborate leukotoxin in a supernate; the culturing is preferably carried out at a temperature of from about 35.degree.-41.degree. C., a pH of from about 6.5-8, and for a period of from about 4-9 hours. At the end oil of the culturing, bacterial growth and leukotoxin elaboration are terminated, preferably by separating the leukotoxin supernate, whereupon the vaccine is produced by inactivation of at least the supernate.
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Chengappa Muckatira M.
Nagaraja Tiruvoor G.
Kansas State University Research Foundation
Lilling Herbert J.
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