Fusobacterium leukotoxoid vaccine

Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Bacterium or component thereof or substance produced by said...

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4241301, 4241641, 4241761, 4241841, 42419711, 435 713, 435243, 4352521, 435822, A61K 3900, A61K 3902

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active

054926947

ABSTRACT:
A method is provided for the enhanced elaboration of leukotoxin from F. necrophorum, and subsequent production of an inactivated leukotoxoid ruminant animal vaccine against F. necrophorum infection and consequent liver abscesses and/or foot rot in such animals. The method involves forming a culture of F. necrophorum bacteria in growth media, allowing the bacteria to grow therein and to simultaneously elaborate leukotoxin in a supernate; the culturing is preferably carried out at a temperature of from about 35.degree.-41.degree. C., a pH of from about 6.5-8, and for a period of from about 4-9 hours. At the end of the culturing, bacterial growth and leukotoxin elaboration are terminated, preferably by separating the leukotoxin supernate, whereupon the vaccine is produced by inactivation of at least the supernate.

REFERENCES:
patent: 4152415 (1979-05-01), Harris et al.
patent: 4919929 (1990-04-01), Beck
Emery et al "Vet. Microbiol." 12, 255-268 (1986) Generation of Immunity Against Fusobacterium Necrophorum in Mice Inocculated with Extracts Containing Leucocidin.
Emery et al "Australian Vet J." (61): 382-385 (1984) Biochemical and Functional Properties of a Leucocidin Produced by Several Strains of Fusobacterium Necrophorum.

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