Fusion proteins, their preparation and use

Details Fusion proteins, their preparation and use Fusion proteins, their preparation and use

1992-04-23

1993-07-13

Hill, Jr., Robert J.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 691, 4351723, C12N 1512, C12N 1562

Patent

active

052272931

ABSTRACT:
Fusion proteins are obtained in high yields if a mixed oligonucleotide is constructed which codes for the ballast constituent of the fusion protein. The oligonucleotide mixture is introduced in a vector in such a manner that it is functionally linked to a regulatory region and to the structural gene for the desired protein. Appropriate host cells are transformed with the plasmid population obtained in this manner and the clones producing a high yield of coded fusion protein are selected.

REFERENCES:
patent: 4366246 (1982-12-01), Riggs
patent: 4431739 (1984-02-01), Riggs
patent: 4652639 (1987-03-01), Stabinsky
patent: 4769326 (1988-09-01), Rutter
Oliphant, A. R. et al., Gene, 44:177-183 (1986).
Butt, T. R., et al., Proc. Natl. Acad. Sci. USA, 86:2540-2544 (1989).
Bachmair, A. et al., Science, 234:179-186 (1986).
Reidharr-Olson, J. F. et al., Science, 241:53-57 (1988).
Saiki, R. K. et al., Science, 239:487-491 (1988).
Aota, S. et al., Nucl. Acids Res., 16 (suppl.):r315-r316, r391 and r402 only (1988).
Helfman, D. M. et al., Proc. Natl. Acad. Sci. USA, 80:31-35 (1983).
Ho, S. N. et al., Gene, 77:51-59 (1989).

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