Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease
Reexamination Certificate
2007-07-17
2007-07-17
Rao, Manjunath N (Department: 1652)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Transferase other than ribonuclease
C435S004000, C435S006120, C435S069100, C435S183000, C435S194000, C435S252300, C435S320100, C530S350000, C536S023200, C536S023400
Reexamination Certificate
active
09211691
ABSTRACT:
This invention provides fusion polypeptides that include a glycosyltransferase catalytic domain and a catalytic domain from an accessory enzyme that is involved in making a substrate for a glycosyltransferase reaction. Nucleic acids that encode the fusion polypeptides are also provided, as are host cells for expressing the fusion polypeptides of the invention.
REFERENCES:
patent: 5032519 (1991-07-01), Paulson et al.
patent: 5352670 (1994-10-01), Venot et al.
patent: 5374541 (1994-12-01), Wong et al.
patent: 5516665 (1996-05-01), Wong
patent: WO 96 21038 (1996-07-01), None
patent: WO 96 32491 (1996-10-01), None
patent: WO 96 35801 (1996-11-01), None
patent: WO 96 40971 (1996-12-01), None
patent: WO 98 07837 (1998-02-01), None
Ito et al., “Synthesis of bioactive sialosides,”Pure&Appl. Chem. 65(4):753-762 (1993)..
Aoki et al., Analysis of the substrate binding sites of human galactosyltransferase by protein engineering,EMBO. J. 9: 3171-3178 (1990).
Harduin-Lepers et al., “Mini Review 1994, the year of the sialyltransferases,”Glycobiology5(8): 741-758, (1995).
Natsuka and Lowe, “Enzymes involved in mammalian oligosaccharide biosynthesis,”Curr. Opin. Struct. Biol. 4: 683-691, (1994).
Zu et al., “Use of Site-Directed Mutagenesis to Identify the Galactosyltransferase Binding Sites for UDP-Galactose,”Biochem. Biophys. Res. Comm. 206(1): 362-369 (1995).
Seto et al., “Expression of a recombinant human glycosyltransferase from a synthetic gene and its utilization for synthesis of the human blood group B trisaccharide,”Eur. J. Biochem. 234: 323-328 (1995).
Seto et al., “Sequential Interchange of Four Amino Acids from Blood Group B to Blood Group A Glycosyltransferase Boosts Catalytic Activity and Progressively Modifies Substrate Recognition in Human Recombinant Enzymes,”Eur. J. Biochem272: 14133-141388 (1997).
Gilbert et al., “Purification and characterization of the recombinant CMP-sialic acid synthetase fromNeisseria meningitidis,” Biotechnol. Lett. 19: 417-420 (1997).
Gilbert et al., “Cloning of the Lipooligosaccharide a-2-3-Sialyltransferase from the Bacterial PathogensNeisseria meningitidisandNeisseria gonorrhoeae,” J. Biol. Chem. 271: 28271-28276 (1996).
Gilbert et al., “Characterization of a recombinantNeisseria meningitidisa-2,3-sialyltransferase and its acceptor specificity,”Eur. J. Biochem. 249: 187-194 (1997).
Gilbert et al., “The synthesis of sialylated oligosaccharides using a CMP-Neu5Ac synthetase/sialyltransferase fusion,”Nature Biotechnol.. 16: 769-772 (1998).
Wakarchuk et al., “Functional Relationships of the Genetic Locus Encoding the Glycosyltransferase Enzymes Involved in Expression of the Lacto-N-neotetraose Terminal Lipopolysaccharide Structure inNeisseria meningitidis,” J. of Biol. Chem. vol. 271, No. 32: 19166-19173 (1996).
Stevenson et al., “Organization of theEscherichia coliK-12 Gene Cluster Responsible for Production of the Extracellular Polysaccharide Colanic Acid,”J. Bacteriol. 178: 4885-4893, (1996).
Bülow, L. and Mosbach, K. “Multienzyme systems obtained by gene fusion,”TIBTECHJul. 1991, pp. 226-231, vol. 9.
Guo, Z. and Wang, P.G. “Utilization of glycosyltransferases to change oligosaccharide structures,”Appl. Biochem. &Biotechnol. 1997, pp. 1-20, vol. 68.
Fang et al., “A unique chemoenzymatic synthesis of α-galactosyl epitope derivatives containing free amino groups: Efficient separation and further manipulation,” J. Org. Chem. 1999, pp. 4089-4094, vol. 64.
Chen et al., “Changing the donor cofactor of bovine α1,3-galactosyltransferase by fusion with UDP-galactose 4-epimerase,” J. Biol. Chem. 2000, pp. 31594-31600, vol. 275.
Warner, Thomas G., “Sweet success with tethered enzyme catalysis,” Nature Biotechnology, Aug. 1998, pp. 720-721, vol. 16.
Gilbert Michel
Wakarchuk Warren W.
Young N. Martin
National Research Council of Canada
Rao Manjunath N
Townsend and Townsend / and Crew LLP
LandOfFree
Fusion proteins for use in enzymatic synthesis of... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Fusion proteins for use in enzymatic synthesis of..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Fusion proteins for use in enzymatic synthesis of... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3756587