Fusion proteins, DNA molecules, vectors, and host cells useful f

Details Fusion proteins, DNA molecules, vectors, and host cells useful f Fusion proteins, DNA molecules, vectors, and host cells useful f

1998-08-31

2000-09-12

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 71, 435455, 435465, 435471, C12Q 168, G01N 3353, C12N 1587, C12N 1574

Patent

active

061176397

ABSTRACT:
The present invention relates to novel fusion proteins, DNA molecules encoding the same, vectors comprising the DNA molecules, and host cells containing the vectors for use in measuring protease activity using a novel transcriptional assay. This invention also relates to a method for determining the inhibitory activity of a compound against a protease and to a method for comparing the activity of two proteases which recognize the same cleavage site. Kits for assaying protease activity comprising DNA molecules encoding the fusion protein substrates of this invention are also contemplated.

REFERENCES:
patent: 5599906 (1997-02-01), Dasmahapatra
patent: 5861267 (1999-01-01), Su
David No, et al., "Ecdysone-Inducible Gene Expression in Mammalian Cells and Transgenic Mice," Proc. Natl. Acad. Sci. USA, 93, pp. 3346-3351 (1996).
D. Latchman, "Eukaryotic Transcription Factors," Academic Press, San Diego, pp. 81-89, 95-99, 271-273, 298.
Bimalendu Dasmahapatra, et al., "A Genetic System for Studying the Activity of a Proteolytic Enzyme," Proc. Natl. Acad. Sci, 89, pp. 4159-4162 (1992).
Michael G. Murray, et al., "Inactivation of a Yeast Transactivator by the Fused HIV-1 Proteinase: a Simple Assay for Inhibitors of the Viral Enzyme Activity," Gene, 134, pp. 123-128 (1993).
Yuji Hirowatari, et al., "A Novel Method for Analysis of Viral Proteinase Activity Encoded by Hepatitis C Virus in Cultured Cells," Analytical Biochemistry, 225, pp. 113-120 (1995).
Tracy A. Smith, et al., "Direct Selection for Sequences Encoding Proteases of Known Specificity," Proc. Natl. Acad. Sci., 88, pp. 5159-5162 (1991).

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