Fusion proteins comprising cell cycle regulatory proteins

Details Fusion proteins comprising cell cycle regulatory proteins Fusion proteins comprising cell cycle regulatory proteins

1996-12-19

1998-09-29

Cunningham, Thomas M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Stablizing an enzyme by forming a mixture, an adduct or a...

435 697, 435194, 530350, 530358, 536 234, C12N 912, C12N 1552

Patent

active

058145036

ABSTRACT:
The present invention provides novel fusion proteins comprising cyclins and CDKs. A preferred embodiment of the invention provides fusion proteins comprising human cyclin D1 and human CDK4. The fusion proteins of the invention optionally contain modifications, which facilitate their purification. Addition of histidine residues to selected constructs allows purification via immobilized metal affinity chromatography. Antigenic determinants allowing monoclonal antibody-based affinity chromatography purification are provided in selected embodiments of the invention. Protease cleavage sites are incorporated in selected constructs to allow cleavage of the regions incorporated in the cyclin-CDK fusion proteins for purification. Additional modifications which facilitate purification include strepavadin binding domains and antigenic determinants for antibody affinity chromatography.

REFERENCES:
Gadbois et al. 1995 Exp. Cell Research 220: 220-225.

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