Fusion proteins

Details Fusion proteins Fusion proteins

2003-01-10

2008-03-25

Mondesi, Robert B. (Department: 1652)

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Reexamination Certificate

active

07348408

ABSTRACT:
The present invention relates to fusion proteins (fusion polypeptides), particularly for use in expression and/or purification systems. The present inventors have found that the TolAIII domain has remarkable properties which are of particular use as a fusion protein partner to achieve high levels of expression in a host cell. In one aspect of the invention, a TolAIII domain or a functional homologue, fragment, or derivative thereof is located towards the N-terminus of the fusion polypeptide and a non-TolA polypeptide is located towards the C-terminus of the fusion polypeptide.

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Riechmann et al, CELL, vol. 90, pp. 351-360, Jul. 25, 1997, The C-Terminal Domain of TolA is the Coreceptor for Filamentous . . . .
Lubkowski et al, STRUCTURE, vol. 7, No. 6, 1999, pp. 711-722, Filamentous phage infection: crystal structure of g3p in . . . .
Derouiche et al, BIOLOGY 143, 1997, pp. 3185-3192, Binding of Colicins A and E1 to purified ToIA domains.
Wan et al, Protein Expression & Purification 14, pp. 13-22, 1998, ToIAIII Co-overexpression Facilitates the Recovery of . . . .
LaVallie et al, Bio/Technology, vol. 11, pp. 187-193, Feb. 11, 1993, A Thioredoxin Gene Fusion Expression System that . . . .
Deprez et al, Biochemistry 41, 2002, pp. 2589-2598, Macro-molecular Import intoEscherichia coli: The ToIA C-Terminal . . . .
Walburger et al, Molecular Microbiology 44 (3), pp. 695-708, 2002, The ToI/PaI system function requires an interaction . . . .
Anderluh et al, Protein Expression & Purification 28, pp. 173-181, 2003, Expression of proteins using the third domain of . . . .
Gokce et al, J Mol Biol 304, 2000, pp. 621-632, The ToIA-recognition Site of Colicin N. ITC, SPR, and Stopped-flow . . . .

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