Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Patent
1994-05-02
1996-12-31
Wax, Robert A.
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
4352523, 4353201, 435 693, 4351723, 435 697, 4352401, 43525411, 536 234, 530350, 530802, 530808, C12N 1561, C12N 100, C12N 121, C12N 1563, C07K 1428, C07K 1424
Patent
active
055893848
ABSTRACT:
A fusion protein suitable for use as a vaccine comprises an amino acid sequence having biological activity which is fused via an intervening hinge comprising from two to eight glycine-proline repeats to the C-terminus of sufficient of the amino acid sequence of a B subunit of an enterotoxin which is capable of ADP-ribosylation of a GTPase.
REFERENCES:
patent: 4898818 (1990-02-01), Nakai et al.
Lipscombe (1991) "Construction and characterisation of Escherichia coli heat labile toxin B-subunit fusion proteins (enterotoxin)" Ph.D. Dissertation, U. Warwick, U.K.
Nashar et al, "Current progress in the development of the B subunits of cholera toxin and Eschericihi coli heat-labile enterotoxin as carriers for the oral delivery of heterologous antigens and epitopes", Vaccine 11:235-240 (1993).
Hirst, "Assembly and Secretion of Oligomeric Toxins by Gram-Negative Bacteria", Sourcebook of Bacterial Protein Toxins, Chapter 5, pp. 75-100 (1991) London: Academic Press.
Fergusson et al, "A Multivalent Carrier for Delivery of Epitopes and Anitgens Based upon the B-subunit Enterotoxoid of Eschericiha coli", Bacterial Protein Toxins, Zbl. Bakt. Suppl. 19, Rappuoli et al (Eds.), pp. 519-520 (1990).
Spangler, "Structure and Function of Cholera Toxin and the Related Escherichia coli Heat-Labile Enterotoxin", Microbiological Reviews 56(4):622-647 (1992).
Guzman-Verduzco et al J. Bacteriol 169, No. 11 5201-5208, Nov. 1987 "Fusion of Escherichia coli Heat-Stable . . . Subunit".
Purvis et al J. Mol. Biol. 193, 413-417, 1987 "The efficiency of Folding of Some Proteins is Increased . . . Translation in Vivo".
Sandkvist et al. J. Mol. Biol. 169, 4570-4576 1987 "Alterations at the Carboxyl Terminus . . . Enterotoxin".
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Dertzbaugh et al, Infect. Immun. 58, 70-79, 1990 "Cholera Toxin B-subunit Gene Fusion: Structural and Functional . . . Chimeric Protein".
Schodel et al, Infect. Immun. 57, 1347-1350, 1989 "Construction of a Plasmid for Expression of Foreign Epitopes . . . Enterotoxin".
van Heyningen, Nature 351, 351, May 1991 "Enterotoxins The ring on a Finger".
Sixma, Nature 351, 371-377, May 1991 "Crystal structure of a cholera toxin-related . . . E. coli".
Lipscombe et al, Mol. Microbiol. 5, 6, 1385-1392, 1991 "Intranasal immunization using the B subunit . . . antigen".
Clements, Infect. Immun 58 1159-1166, 1990 "Construction of a Nontoxic Fusion Peptide for Immunization . . . Enterotoxins".
Daum et al, "Production of human adrenocorticotropin by cleavage of alkaline-phosphatase-derived fusion proteins containing repetitive recognition sequences for collagenases", Eur. J. Biochem. 185 3/7 (1989).
Charles Ian G.
Fairweather Neil F.
Lipscombe Martin J.
Bugaisky Gabriele E.
Glaxo Wellcome Inc.
Wax Robert A.
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