Fusion protein incorporating a cysteinized ribonuclease

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

Reexamination Certificate

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C435S069700, C435S196000, C435S069100, C435S320100, C435S325000, C435S252300, C435S019000, C530S350000, C536S023200

Reexamination Certificate

active

07442535

ABSTRACT:
A fusion protein contains a cysteinized variant of an RNase disclosed in U.S. Pat. No. 6,239,257 B1.

REFERENCES:
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patent: 2003/0099629 (2003-05-01), Goldenberg et al.
patent: 00/40608 (2000-07-01), None
patent: 01/18214 (2001-03-01), None
patent: 2004/061120 (2004-07-01), None
Branden et al., Introduction to Protein Structure, Garland Publishing Inc., New York, p. 247, 1991.
Seffernick et al., J. Bacteriol. 183(8):2405-2410, 2001.
Witkowski et al., Biochemistry 38:11643-11650, 1999.
Lehninger, A.L. (1975) Biochemistry 2nd Ed., p. 962.
Studier, F.W. et al (1990) Met. Enzym. 185, 60-89.
Huang, H-C. et al (1998) J. Biol. Chem. 273(11), 6395-6401.
Guerrero, S.A. et al (2000) Appl Microbiol Biotechnol 53, 410-414.
PCT International Preliminary Report on Patentability, PCT/US04/14844 (May 12, 2004), completed Feb. 15, 2007.
Notomista E. et al “Effective expression and purification of recombinant onconase, an antitumor protein” FEBS Letters 463 (1999) 211-215.
Supplementary European Search Report in European application No. EP 04 75 1988, completed May 30, 2007 and mailed Jun. 6, 2007.

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