Fused proteins comprising glycoprotein gD of HSV-1 and LTB

Details Fused proteins comprising glycoprotein gD of HSV-1 and LTB Fused proteins comprising glycoprotein gD of HSV-1 and LTB

1990-09-05

1993-08-31

Lee, Lester L.

Drug, bio-affecting and body treating compositions

Lymphokine

530300, 530350, 530403, 530405, 435 693, 435 697, 536 2372, 424 92, C07K 300, C07K 1300, C07K 1500, A61K 3702

Patent

active

052410536

ABSTRACT:
Disclosed are (1) a fused protein comprising heat-labile enterotoxin B subunit and a protein heterologous to heat-labile enterotoxin, (2) a recombinant DNA containing a nucleotide sequence coding for the above fused protein, (3) a transformant harboring the above recombinant DNA, (4) a method for producing the fused protein which comprises cultivating the above transformant, producing and accumulating the above fused protein in a culture, and collecting the fused protein, and (5) a method for purifying a fused protein comprising a herpes simplex virus surface antigen and heat-labile enterotoxin B subunit, which comprises cultivating a transformant harboring a recombinant DNA containing a nucleotide sequence coding for the fused protein, producing an accumulating the fused protein in a culture, collecting the fused protein and subjecting the collected fused protein to purification processes comprising cationic exchange chromatography and gel permeation chromatography.

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Yamamoto, et al., Molecular Organization of Heat-Labile Enterotoxin Genes Originating in Escherichia coli of Human Origin and Construction of Heat-Labile Toxoid-Producing Strains, Journal of Bacteriology, vol. 148, No. 3, pp. 983-987 (1981).
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