Fused protein for enzyme immunoassay system

Details Fused protein for enzyme immunoassay system Fused protein for enzyme immunoassay system

1986-05-28

1988-05-17

Marantz, Sidney

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 14, 435 68, 435 70, 4351723, 435188, 435810, 435320, 530350, 935 10, 935 11, 935 29, 935 47, G01N 33535, C12N 1500

Patent

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047450550

ABSTRACT:
A fused protein for use in an enzyme immunoassay system. The protein comprises an enzymatically active .beta.-galactosidase fused, at its C terminus, to an immunologically active peptide. The protein is produced using a plasmid containing a complete .beta.-galactosidase gene fused, at its 3' end, with an oligonucleotide coding for the peptide. The fused protein is designed for use in a solid-phase enzyme immunoassay system, based on immunospecific binding of the fused protein to a solid support, or in a homogeneous enzyme immunoassay system, based on enzyme inhibition resulting from immunospecific binding of an antibody to the protein.

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