Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Lymphokines – e.g. – interferons – interlukins – etc.
Patent
1985-05-09
1989-11-14
Hall, Blondel
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Lymphokines, e.g., interferons, interlukins, etc.
530350, 530324, 530399, 424 854, C07K 1526, C07K 1500
Patent
active
048809110
ABSTRACT:
Fused-Polypeptides comprising a polypeptide product and a covalently bonded charged amino acid polymer are disclosed. Methods of detecting the presence of the charged amino acid polymer are used to quantitate the presence of the fused-polypeptides either in crude or purified solutions. The charged amino acid polymer is hydrolyzed using an exopeptidase which leaves the polypeptide product. Examples using polyarginine and carboxypeptidase B to produce urogastrone and gamma interferon are included.
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Brewer Stephen J.
Sassenfeld Helmut M.
G. D. Searle & Co.
Hall Blondel
Kanady Mary Jo
Matukaitis Paul D.
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