Fused liposome and acid induced method for liposome fusion

Details Fused liposome and acid induced method for liposome fusion Fused liposome and acid induced method for liposome fusion

1984-04-19

1988-12-06

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

4352401, 435243, 4352521, 435254, 435257, 4284222, 264 41, 264 43, 264 46, 264 71, 424417, 424420, 424450, C12N 500, C12N 502, B32B 516, A61J 500

Patent

active

047896337

ABSTRACT:
Liposomes containing phosphatidylethanolamine, palmitoyl homocysteine or oleic acid or palmitic acid, fuse rapidly when the pH of the medium is reduced below 7. Liposome fusion was measured by (a) mixing of the liposomal lipids as shown by resonance energy transfer, (b) gel filtration and (c) electron microscopy. The presence of phosphatidylethanolamine or acid addition esters thereof in the liposomes greatly enhances fusion; whereas the presence of phosphatidylcholine inhibits fusion. During fusion of liposomes containing phosphatidylethanolamine:palmitoyl homocysteine (8:2), almost all of the encapsulate calcein is released. Inclusion of cholesterol (40%) in the liposomes substantially decreases leakage without impairing fusion. Those pH sensitive liposomes are fused to deliver biologically active molecules such as DNA, into living cells.

REFERENCES:
patent: 4235871 (1980-11-01), Papahadjopoulos et al.
patent: 4241046 (1980-12-01), Papahadjopoulos et al.
patent: 4394448 (1983-07-01), Szoka, Jr. et al.
D. Papahadjopoulos, Ann. N.Y. Acad. Sci., 308, 1 (1978).
Ann. Rpts. Med. Chem., 14, 250 (1979).
Batzri et al., Biochim. Biophys. Acta, 298, 1015 (1973).
Deamer et al., Biochim. Biophys. Acta, 443, 629 (1976).
Schieren et al., Biochim. Biophys. Acta, 542, 137 (1978).
Razin, Biochim. Biophys. Acta, 265, 241 (1972).
Matsumato et al., J. Colloid Interface Sci., 62, 149 (1977).
Szoka, Jr. et al., Proc. Natl. Acad. Sci. USA, 75, 4194 (1978).
Olson et al., Biochim. Biophys. Acta, 557, 9 (1979).
Magee & Miller, Nature, 235, 339 (1972).
Gregoriadis & Buckland, Nature, 244, 170 (1973).
Papahadjopoulos, Biochim. Biophys. Acta, 323, 23 (1973).
Papahadjopoulos et al., Nature, 252, 163 (1974).
Papahadjopoulos et al., Biochim. Biophys. Acta, 363, 404 (1974).
Papahadjopoulos et al., Cancer Res., 36, 4406 (1976).
Marsh et al., Cold Spring Harbor Symp. Quant. Biol., 46, 835 (1982).
White et al., J. Cell Biol., 89, 674 (1981).
Schneider et al., Natl. Acad. Sci. USA, 77, 442-446 (1980).
Schenkman et al., Chem. Phys. Lipids, 649, 633 (1981).
Schenkman et al., Chem. Phys. Lipids, 28, 165 (1981).
Chaimovich et al., Biophys. J., 41, 28a (1983).
Blumenthal et al., Biol. Chem., 258, 3409 (1983).
Allens et al., J. Cell. Biol., 97, 10 (a), Abstr. No. 419 (1983).
Straubinger, J. Cell Biol., 97, 109(a), Abstr. No. 420 (1983).
White et al., Proc. Natl. Acad. Sci. USA, 77, 3272 (1980).
Marsh et al., J. Cell. Biol., 96, 455 (1983).
Baernstein J. Biol. Chem., 106, 451 (1934).
Yatvin et al., Science, 210, 1253 (1980).
Cestaro et al., J. Biochem., 133, 229 (1983).
Yatvin et al., in Liposome Technology, Gregoriadis, G. (ed.), CRC Press, Boca Raton (1984).
Sundler et al., Biochim. Biophys. Acta, 649, 751 (1981).
Cullis et al., Nature (Lond.), 271, 672 (1978).
Cullis et al., Biophys. Acta, 559, 399 (1979).
Huang et al., J. Biol. Chem., 258, 14034 (1983).
Heath et al., Proc. Natl. Acad. Sci. USA, 80, 1377 (1983).
Leserman et al., Nature (Lond.), 293, 226 (1981).
Machy et al., J. Immunol., 129, 2098 (1982).
Straubinger et al., Cell, 32, 1069 (1983).
Tycko et al., Cell, 28, 643 (1980).
Struck et al., Biochem., 20, 4093 (1981).
Allen et al., Biochim. Biophys. Acta, 597, 418 (1980).
Philippot et al., Biochim. Biophys. Acta, 716, 140 (1982).
Shen et al., Biochim. Biophys. Acta, 689, 31 (1982).
Szoka, Jr. et al., Biochem. Biophys. Res. Comm., 110 (1983).
Huang et al., Biochem. Biophys. Acta, 716, 140 (1982).
Ellens et al., Biochem., 23, 1532 (1984).
Eidelman et al., J. Biol. Chem., 259, 4622 (1984).
Ellens et al., Journal of Cell Biology, vol. 97, p. 109(a), Abstract No. 419.

Affiliated with

Also associated with

Rating

Fused liposome and acid induced method for liposome fusion does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Fused liposome and acid induced method for liposome fusion, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Fused liposome and acid induced method for liposome fusion will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1493900