Fused liposome and acid induced method for liposome fusion

Details Fused liposome and acid induced method for liposome fusion Fused liposome and acid induced method for liposome fusion

1992-02-14

1994-02-01

Lovering, Richard D.

Stock material or miscellaneous articles

Coated or structually defined flake, particle, cell, strand,...

Particulate matter

264 41, 264 43, 264 46, 424450, 4351723, 436829, 935 54, A61K 9127

Patent

active

052831221

ABSTRACT:
Liposomes containing phosphatidylethanolamine, palmitoyl homocysteine or oleic acid or palmitic acid, fuse rapidly when the pH of the medium is reduced below 7. Liposome fusion was measured by (a) mixing of the liposomal lipids as shown by resonance energy transfer, (b) gel filtration and (c) electron microscopy. The presence of phosphatidylethanolamine or acid addition esters thereof in the liposomes greatly enhances fusion; whereas the presence of phosphatidylcholine inhibits fusion. During fusion of liposomes containing phosphatidylethanolamine:palmitoyl homocysteine (8:2), almost all of the encapsulated calcein is released. Inclusion of cholesterol (40%) in the liposomes substantially decreases leakage without impairing fusion. Those pH sensitive liposomes are fused to deliver biologically active molecules such as DNA, into living cells.

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patent: 4534899 (1985-08-01), Sears
patent: 4619794 (1986-10-01), Hauser
patent: 4789633 (1988-12-01), Huang et al.

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