Fused gene and method of making and using same

Details Fused gene and method of making and using same Fused gene and method of making and using same

1979-01-12

1982-06-22

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 91, 435 68, 435207, 536 27, C12N 1500

Patent

active

043363369

ABSTRACT:
This invention is a method for fusing, within a bacterial host, a gene for a cytoplasmic protein to a gene for a non-cytoplasmic protein, so that the hybrid protein produced is transported to, near, or beyond the cell surface for ease of collection and purification, or for immunological use. The invention also encompasses the fused gene created by the disclosed method.

REFERENCES:
Hofnung, Genetics, vol. 76, pp. 169-184, (1974).
Hofnung, Mol. Gen. Genet., vol. 112, pp. 117-132, (1971).
Silhavy et al., Proc. Nat. Acad. Sci., vol. 73, No. 10, pp. 3423-3427, (1976).
Villa-Komaroff et al, Proc. Nat. Acad. Sci., vol. 75, No. 8, pp. 3727-3731, (Aug. 1978).
Casadaban, J. Mol. Biol. 104, pp. 541-555, (1976).
Randall-Hazelbauer et al., J. Bact., vol. 116, pp. 1436-1446, (1973).

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