Fused cell line and method of obtaining the same

Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of...

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435440, C12N 508

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active

060135182

ABSTRACT:
Provided are a human immunocompetent fused cell line, characterized in that any one of a mutant cell selected from the group consisting of ICLU-B, ICLU-T and ICLU-E is used as a parent cell line, wherein said mutant cell is obtained by selecting an 8-azaguanine- or 6-thioguanine-resistant clone from a human Burkitt lymphoma cell line Raji, a human T-cell lymphocytic leukemia cell line PEER and a human eosinophilic leukemia cell line EoL-1, and then treating said clone in order to capable of serum-free culture, and a method of obtaining said human immunocompetent fused cell line.
The human immunocoipetent fused cell line obtained by the present invention is used to investigate food functions, novel drugs and production thereof with the view to the elucidation of mechanisms of phenomena of an immune system such as allergies, cancers and the like using human cell lines, and the prevention, diagnosis and therapy of these diseases.

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