Cleaning compositions for solid surfaces – auxiliary compositions – Cleaning compositions or processes of preparing – For cleaning a specific substrate or removing a specific...
Patent
1997-10-27
1999-11-23
Lilling, Herbert J.
Cleaning compositions for solid surfaces, auxiliary compositions
Cleaning compositions or processes of preparing
For cleaning a specific substrate or removing a specific...
510320, 510321, 435198, 435925, D06L 1600, C11D 742, C12N 920
Patent
active
059900695
DESCRIPTION:
BRIEF SUMMARY
The present invention relates to a novel, biologically pure culture of an isolate of the genus Fusarium, lipolytic enzymes, lipases, cutinases and enzyme compositions derived therefrom and, in particular, to a biologically pure culture of Fusarium solanii var. minus T.92.637/1, lipolytic enzymes, lipases, cutinases and enzyme compositions derived therefrom and the use of such enzymes in detergent compositions.
Lipolytic enzymes, including lipases and cutinases, are commonly employed in detergent cleaning compositions for the removal of fatty acid-based dirt and stains. As such, they have to be stable in presence of these detergents, and in particular in alkaline detergent solutions. Further, lipolytic enzymes are often used in combination with other enzymes, such as alkaline proteases, amylases, oxidases and/or other proteins, thereby requiring them to also be stable in presence of these enzymes.
Lipolytic enzymes naturally produced by various fungi are known.
European Patent Application 0 130 064 describes an enzymatic detergent additive including a lipase naturally produced by the fungal strain Fusarium oxysporum.
International Patent Application WO 90/09446 discloses an enzymatic detergent composition which includes a cutinase naturally produced by Fusarium solanii var. pisi.
To the best of our knowledge, we are not aware of any other lipolytic enzyme naturally produced by any other strains of Fusarium. Further, we are not aware of either any lipase or cutinase which has been naturally produced by any other species of Fusarium, including any isolate of the species Fusarium solanii var. minus.
It is a primary object of the present invention is to provide novel lipolytic enzymes naturally produced by a fungus of the species Fusarium solanii. By the term "lipolytic enzyme" (E.C. 3.1.1), what is meant herein is a lipase (E.C. 3.1.1.3) or a cutinase (E.C. 3.1.1.50) which is capable of removing stains of a fatty nature. Lipase has higher selectivity toward long chain triglycerides contained in fat than cutinase. Cutinase has higher selectivity toward short chain triglycerides contained in fat than lipase.
It is a primary object of the present invention to provide novel lipases which, when incorporated in a detergent composition, are capable of removing fatty acid based dirt and stains from fabrics.
It is another primary object of the present invention to provide a novel cutinase which, when incorporated in a detergent composition, is capable of removing fatty acid based dirt and stains from fabrics.
Still another primary object of the present invention is to provide novel enzyme compositions of lipases and/or cutinases which, when incorporated in a detergent composition, are capable of removing fatty acid based dirt and stains from fabrics.
A further primary object of the present invention is to identify, isolate and provide a biologically pure culture of a novel fungus of the genus Fusarium which is capable of naturally producing the lipolytic enzymes, including the lipases and cutinases, of the present invention which lipolytic enzymes may be incorporated into the enzymatic compositions and/or the detergent compositions of the present invention.
A yet further primary object of the present invention is to provide a method for removing fatty acid-based dirt and stains from fabrics with the use of the enzymes and/or the enzyme compositions of the present invention.
A still yet further object of the present invention is to provide an enzymatic detergent composition capable of removing fatty acid-based dirt and stains from fabrics, which detergent composition includes a lipolytic enzyme and/or a lipase and/or cutinase and/or enzyme composition.
In accordance with the teachings of the present invention, disclosed herein is a biologically pure culture of the novel isolate Fusarium solanii var. minus T.92.637/1 and mutants and derivatives thereof. This isolate is capable of producing the lipolytic enzymes, the lipases, the cutinases and the enzyme compositions of the present invention.
In another aspect of the pres
REFERENCES:
Chander & Klostermeyer, "Production of lipase by Fusarium solani under various growth conditions," Sciences Des Aliments, pp. 279-285 (1983).
Flurkey W.H. et al., "In vitro translation of cutinase mRNA:evidence for a precursor form of an extracellular fungal enzyme," Archives of Biochemistry and biophysics, vol. 212(1):154-161 (1981).
Koller W. et al., "Mechanism of Action of Cutinase Chemical Modification of the Catalytic Triad Characteristic for Serine Hydrolases," Biochemistry, vol. 21(13):3083-3090 (1982).
Martinez et al., "Fusarium solani cutinase is a lipolytic enzyme with a catalytic serine accessible to solvent," Nature, vol. 356 (6370):615-618 (1992).
Purdy & Kolattukudy, "Hydrolysis of plant cuticle by plant pathogens. Purification, amino acid composition and molecular weight of two isozymes of cutinase and a nonspecific esterase from Fusarium solani f. pisi," Biochemistry, vol. 14, No. 13 (1975).
Purdy et al., "Depolymeriztion of a hydroxy fatty acid biopolymer, cutin, by an extracellular enzyme from Fusarium solani f. pisi Isolation and some properties of the enzyme," Arch. biochem. Biophys., vol. 159(1):61-69 (1973).
Soliday et al., "Isolation and characterization of a cutinase from Fusarium roseum culmorum and its immunological comparison with cutinases from F. solani pisi," Archives of Biochemistry and Biophysics, vol. 176:334-343 (1976).
Soliday et al., "Cloning and structure determination of cDNA for cutinase, an enzyme involved in fungal penetration of plants," Proc. Natl. Acad. Sci. USA, vol. 81:3939-3943 (1984).
Sreekantiah et al., "The production of certain extracellular hydrolytic enzymes by four species of plant pathogenic fungi," Indian Journal of Microbiology, vol. 12:71-78 (1972).
Andre Christophe
Charmoille Lucien
Faris Susan
Genencor International Inc.
Lilling Herbert J.
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