Fungus extraction method, kit, and extraction solution

Details Fungus extraction method, kit, and extraction solution Fungus extraction method, kit, and extraction solution

1994-07-01

1996-09-24

Housel, James C.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

4351731, 4351737, 435243, 4352541, 435803, 435810, 435961, 435962, 435975, 436174, 436175, 436177, 436178, 436825, 436826, 510199, G01N 3353

Patent

active

055589960

ABSTRACT:
A method and kit for extracting fungus from a plant. An extraction solution is combined with tissue from a plant and the combination is agitated, preferably by shaking, to extract a detectable amount of the fungus from the plant. The extraction solution is a solution containing a dilute acid and a detergent. The extract can then be neutralized without degradation of the fungus and subjected to analysis by immunoassay to detect the presence of pathogenic fungi. The method is particularly useful for the detection of Pyricularia oryzae on rice plants.

REFERENCES:
patent: 4309299 (1982-01-01), Rapisarda et al.
patent: 4650754 (1987-03-01), Brambl
patent: 4693968 (1987-09-01), Kitagawa
patent: 4851337 (1989-07-01), Berke
patent: 5186946 (1993-02-01), Vallieres
Chemical Abstracts, vol. 118, No. 9, p. 638, col. 1, abstract No. 78951; Xia, J. Q. et al., "Monoclonal antibodies to an extracellular component of Pyricularia grisea," Can. J. Bot., 70(9):1790-1797 (1992).
Chemical Abstracts, vol. 108, No. 19, p. 380, col. 2, abstract 164630; Kitagawa, t. et al., "A novel enzyme immunoassay commonly applies for ten strains of Pyricularia oryzae," Microbiol. Immunol., 31(12):1197-1207 (1987).
Xia, J. O., et al., "Development of Monoclonal Antibodies Specific for Pyricularia grisea, the Rice Blast Pathogen," Mycol. Res., 96:867-873 (1992).
C. Merodio et al, "Improved Separation of Green and Soluble Leaf Proteins by pH Shift", Journal of Agricultural Food Chemistry 31(5):956-959, 1983.

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