Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
2006-04-21
2008-05-20
Carlson, Karen Cochrane (Department: 1656)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
C435S235100, C435S253300
Reexamination Certificate
active
07375204
ABSTRACT:
A reporter system reflecting the transport process that transports GPI-anchored proteins to the cell wall was constructed and compounds inhibiting this process were discovered. Further, fungal genes conferring resistance to the above compounds were identified and methods of screening for compounds that inhibit the activity of the proteins encoded by these genes were developed. These genes encode proteins participating in fungal cell wall synthesis. Therefore, through the novel compounds, the present invention showed that antifungal agents having a novel mechanism, i.e. inhibiting the process that transports GPI-anchored proteins to the cell wall, could be achieved.
REFERENCES:
patent: 4013666 (1977-03-01), Lenz
patent: 5641627 (1997-06-01), Moehle
patent: 5648465 (1997-07-01), Margolis et al.
patent: 6747137 (2004-06-01), Weinstock et al.
patent: 2002/0072051 (2002-06-01), Bulawa et al.
patent: 2006/0172404 (2006-08-01), Hata et al.
patent: 2006/0234283 (2006-10-01), Tsukahara et al.
patent: 2006/0240429 (2006-10-01), Tsukahara et al.
patent: WO 94/01567 (1993-07-01), None
patent: WO 99/39197 (1999-08-01), None
patent: WO 00/01387 (2000-01-01), None
White et al. (1998) Clinical, cellular, and molecular factors that contribute to antifungal drug resistance. Clin. Microbiol. Rev. vol. 11, No. 2, pp. 382-402.
Umemura et al. (2003) GWT1 gene is required for inositol acylation of glycosylphosphatidylinositol anchors in yeast. J. Biol. Chem. vol. 278, No. 26, pp. 23639-23647.
Mohuczy et al. (1999) Delivery of antisense DNA by vectors for prolonged effects in vitro and in vivo. Methods Enzymol. vol. 314, pp. 32-51.
Skrzypek et al. (1997) “Suppressor gene analysis reveals an essential role for sphingolipids in transport of glycosylphosphatidylinositol-anchored proteins inSaccharomyces cerevisiae”, J. Bacteriol., vol. 179, No. 5, pp. 1513-1520.
Wiktionary (2007, updated) “transformant”, http://en.wiktionary.org/wiki/transformant, p. 1.
Davila, J.C. et al.; “Cytotoxicity induced by papaverine hydrochloride in fungal cell systems”; 1990,Toxicology Letter, vol. 54, pp. 23-31.
Hamada, K., et al., “Screening for Glycosylphoshpatidylinositol (GPI)-Dependent Cell Wall Proteins inSaccharomyces cerevisiae,” Molecular and General Genetics, Apr. 1998, pp. 53-59, vol. 258, No. 1-2, Springer-Verlag.
Mamyrbekova, Z.A. et al.; “Synthesis, structure, and biological activity of paracyclophane derivatives”; 1995,Chemical Abstracts, vol. 123, p. 592.
Mamyrbekova, Z.A. et al.; 1994,Khim Pharm., vol. 28, No. 3, pp. 48-51.
Miosga, T. et al.; “S. cerevisiaechromosome X reading frame ORF YJL091c”;GenBank Database; Aug. 11, 1997; Accession No. Z49366.
Miosga, et al., “Sequence Analysis Of A 33-1 kb Fragment From The Left Arm OfSaccharomyces cerevisiaeChromosome X, Including Putative Proteins With Leucine Zippers, A Fungal Zn(II)2-Cys6Binuclear Cluster Domain And A Putative α2-SCB-α2 Binding Site”,Yeast, vol. 11: 681-689 (1995).
Prostakov, N.S. et al.; 1980,Chemical Abstractsvol. 93, No. 17, p. 660.
Prostakov, N.S. et al.; 1980,Khim Geterotsikl Soedin, vol. 5, pp. 673-676.
Seow, W.K. et al.; 1993,Chemical Abstracts, vol. 119, No. 23, p. 32.
Seow, W.K. et al.; “Inhibitory effects of bisbenzylisoquinolines on synthesis of the inflammatory cytokines interleukin-1 and tumor necrosis factor-alpha”; 1993,Mediators of Inflammation, vol. 2, pp. 199-203.
Shiotani, Shunsaku et al.; “Wittig-Horner Reaction of a Phosphonate of Reissert Analogues of Furo”; 1997,J. Heterocyclic Chemistry, vol. 34, No. 1, pp. 129-141.
Smirnov, L.D. et al.; 1975,Chemical Abstracts, vol. 82, No. 3, p. 484.
Smirnov, L.D. et al.; 1974,Khim Geterotsikl, vol. 8, pp. 1094-1095.
Tsai, I.L. et al.; 1989,Chemical Abstracts, vol. 111, No. 5, p. 342.
Tsai, I.L. et al.; “Screening of Isoquinoline Alkaloids and their Derivatives for Antibacterial and Antifungal Activities”; 1989,Kalhsiung J. Med. Sci., vol. 5, pp. 132-145.
Tsukahara, K., et al., “Medicinal Genetics Approach Towards Identifying the Molecular Target of a Novel Inhibitor of Fungal Cell Wall Assembly,”Molecular Microbiology, May 2003, pp. 1029-1042, vol. 48, No. 4, Blackwell Publishing Ltd.
Umemura, M., et al., “GWT1Gene Is Required for Inositol Acylation of Glycosylphosphatidylinositol Anchors in Yeast,”Journal of Biological Chemistry, Jun. 2003, pp. 23639-23647, vol. 278, No. 26, The American Society for Biochemistry and Molecular Biology, Inc., U.S.A.
Van Der Vaart, J. M., et al., “Identification of Three Mannoproteins in the Cell Wall ofSaccharomyces cerevisiae,” Journal of Bacteriology, Jun. 1995, pp. 3104-3110, vol. 117, No. 11, American Society for Microbiology.
White, et al., “Clinical, cellular, and molecular factors that contribute to antifungal drug resistance,”Clin. Microbiol. Rev., 1998, vol. 11, pp. 382-402, Review.
Database EMBL Online; Accession No. AL132675; Created on Oct. 27, 1999; Description: S.pombe Chromosome I Cosmid c144, 9 pages.
Attachment 1 (2005) Sequence alignment between nucleotide sequences of instant Seq ID No. 3 and Seq ID No. 998 of U.S. Pat. No. 6,747,137, pp. 1-4.
Sütterlin, C., et al., “Saccharomyces cerevisiaeCGI10, the functional homologue of humanPIG-B, is required for glycosylphosphatidylinositol-anchor synthesis,”Biochem. J., vol. 332, pp. 153-159 (1998).
Miosga, T., et al., “Sequence analysis of a 33.1 kb fragment from the left arm ofSaccharomyces cerevisiaechromosome X, including putative proteins with leucine zippers, a fungal ZN (II) 2-Cys6 binuclear cluster domain and a putative alpha 2-SCB-alpha 2 binding site,” NCBI; Accession No. P47026 (Feb. 1, 1996).
GenBank Accession No. CAB59690; Oct. 1999 McDougall, R.C., et al. Wood, V., et al., “The genome sequence ofSchizosaccharomyces pombe,” 2002,Nature, vol. 415, pp. 871-880.
Ikezawa, H., et al., “Glycosylphosphatidylinositol (GPI)-anchored proteins,” 2002,Biol. Pharm. Bull., vol. 25(4), pp. 409-417.
Wikipedia (2007, updated), “Homology (biology)” http://en.wikipedia.org/wiki/Homology—(biology), p. 1.
Hata Katsura
Kai Junko
Nakamoto Kazutaka
Ohba Fuminori
Sagane Koji
Carlson Karen Cochrane
Eisai R&D Management Co., Ltd.
Liu Samuel Wei
Townsend and Townsend / and Crew LLP
LandOfFree
Fungal cell wall synthesis gene does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Fungal cell wall synthesis gene, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Fungal cell wall synthesis gene will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3986874