Fungal cell wall degrading enzyme

Drug – bio-affecting and body treating compositions – Enzyme or coenzyme containing – Hydrolases

Reexamination Certificate

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Details

C435S018000, C435S195000, C536S023200

Reexamination Certificate

active

07635470

ABSTRACT:
A novel fungal enzyme having lysozyme activity has been isolated. The invention further relates to a fungal polypeptide having lysozyme activity and belonging to the GH25 family, wherein the enzyme is selected from the group consisting of (a) a polypeptide comprising an amino acid sequence, which has at least 80% identity with amino acids 1 to 233 of SEQ ID NO:2; (b) a polypeptide comprising an amino acid sequence, which has at least 80% identity with the polypeptide encoded by the lysozyme encoding part of the nucleotide sequence inserted into a plasmid present in strain DSM 16084; (c) a polypeptide which is encoded by a nucleotide sequence which hybridizes under high stringency conditions with a polynucleotide probe consisting of the complementary strand of nucleotides 84 to 782 of SEQ ID NO:1; or (d) a fragment of (a), (b) or (c) that has lysozyme activity.

REFERENCES:
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patent: 1 068 871 (2001-01-01), None
patent: 2 331 750 (1999-06-01), None
patent: WO 91/06009 (1991-05-01), None
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Felch et al, The Journal of Biological Chemistry, vol. 250, Part 10, pp. 3713-3720 (1974).
Seo et al, Journal of BioScience and Bioengineering, vol. 95, No. 3, pp. 313-316 (2003).
Chen et al, Water Research, vol. 34, No. 17, pp. 4229-4233 (2000).
Parkar et al, Journal Ind. Microbial Biotehnology, vol. 30, pp. 553-560 (2003).
Felch et al, Database UNIPROT, Accession No. P00721 (XP-002291508) (1986).
Internet Article on “Cazy —Carbohydrate-Active Enzymes” (XP-002327845) (2005).

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