Fungal beta-glucuronidase genes and gene products

Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part

Reexamination Certificate

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C435S070100, C435S320100, C435S419000, C435S252300, C536S023740

Reexamination Certificate

active

07148407

ABSTRACT:
Nucleic acid molecules encoding fungal β-glucuronidases are provided. Gene products, expression vectors and host cells suitable for expressing β-glucuronidase are also provided. In addition, uses of the β-glucuronidase as a visual and as a selectable marker for transformation are also described.

REFERENCES:
patent: WO 00/55333 (2000-09-01), None
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Guo et al., Protein Tolerance to Random Amino Acid Change, PNAS., 101:9205-9210, 2004.
Jefferson et al. “Beta-glucuronidase fromEscherichia colias a gene fusion marker.” Proc. Natl. Acad. Sci. USA (Nov. 1986) vol. 83 pp. 8447-8451.
Kuroyama et al., “Purification and characterization of a beta-glucuronidase fromAspergillus niger.” Carb. Res. (Jun. 22, 2001) vol. 333, No. 1, pp. 27-39.
Gallagher et al “TheEscherichia coligus operon: induction and expression of the gus operon inE. coliand the occurrence and use of GUS in other bacteria.” Gus Protocols: Using the Gus gene as a reporter of gene expression. pp. 7-22, 1992.
Wenzl et al. “A Functional Screen Identifies Lateral Transfer of Beta-Glucuronidase (gus) from Bacteria to Fungi.” Mol. Biol. Evol. vol. 22(2) pp. 306-316, 2005.

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