Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2011-06-14
2011-06-14
Gabel, Gailene R (Department: 1641)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S006120, C435S040500, C435S287200, C435S372000, C436S517000, C436S538000, C436S548000, C436S056000, C436S063000, C436S172000, C436S175000, C422S067000, C422S073000
Reexamination Certificate
active
07960131
ABSTRACT:
A method of assaying nucleotide receptor P2X7pore activity in white blood cells contained within a mixed cell sample is provided comprising labeling white blood cells with a white blood cell-specific label; depolarizing the labeled white blood cells with an isotonic depolarizing solution; contacting the labeled white blood cells with dye and a P2X7agonist in an amount sufficient to activate P2X7pore activity; contacting the labeled white blood cells with a divalent cation in an amount sufficient to deactivate P2X7pore activity; and analyzing dye uptake whereby P2X7pore activity is quantified by the amount of dye taken up in labeled white blood cells treated with the P2X7agonist relative to labeled white blood cells in the absence of said P2X7agonist, said P2X7pore activity being corrected for sample age and by subtraction of P2X7pore activity contributed by nonviable white blood cells.
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Bertics Paul J.
Denlinger Loren C.
Hogan Kirk J.
Schell Kathleen
Gabel Gailene R
Quarles & Brady LLP
Wisconsin Alumni Research Foundation
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