Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1992-04-03
1994-10-18
Patterson, Jr., Charles L.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
536 232, 536 234, C12N 922, C12N 1555, C12N 1562
Patent
active
053568028
ABSTRACT:
The present inventor have identified of the recognition and cleavage domains of the FokI restriction endonuclease. Accordingly, the present invention relates to DNA segments encoding the recognition and cleavage domains of the FokI restriction endonuclease, respectively. The 41 kDa N-terminal fragment constitutes the FokI recognition domain while the 25 kDa C-terminal fragment constitutes the FokI cleavage nuclease domain. The present invention also relates to hybrid restriction enzymes comprising the nuclease domain of the FokI restriction endonuclease linked to a recognition domain of another enzyme.
REFERENCES:
Bocklage, H., et al, (1991) Nuc. Acids Res 19(5), 1007-1013.
Kita, K., et al, (1989) J. Biol. Chem. 264(10), 5751-5756.
Looney, M. C., et al, (1989) Gene 80, 193-208.
Li, L., et al (1992) Proc. Natl. Acad. Sci., USA 89, 4275-4279.
Patterson Jr. Charles L.
The Johns Hopkins University
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