Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2006-04-04
2006-04-04
Wax, Robert A. (Department: 1653)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S007200, C435S069100, C435S320100
Reexamination Certificate
active
07022488
ABSTRACT:
The invention resides in part in the discovery that G proteins other than Gα15couples to T1R and T2R taste receptors, particularly Giproteins such as Gαi. Related to this discovery, the invention provides cell-based assay methods for identifying compounds that modulate the activity of specific T1R or T2R taste receptors or which modulate the effect of other T1R or T2R modulators on T1R or T2R activity. These assay methods preferably detect the effect of a putative T1R or T2R modulator compound on MAPK activation, cAMP accumulation, or adenylyl cyclase activity or another signaling pathway regulated by Giproteins. The level of MAPK activation, cAMP accumulation or adenylyl cyclase is preferably determined by immunoassay methods that use ligands (monoclonal or polyclonal antibodies) that specifically bind an activated (phosphorylated) MAPK, cAMP, or adenylyl cyclase.
REFERENCES:
patent: 6558910 (2003-05-01), Zuker et al.
patent: 2002/0051997 (2002-05-01), Zuker et al.
patent: 2002/0160424 (2002-10-01), Adler et al.
patent: 2004/0191862 (2004-09-01), Zoller et al.
McDonald et al., Analytical Biochemistry, 1999, 268, 318-329.
Naor et al., Trends in Endorcinology Metabolism, 2000, 11(3), 91-99.
Wu et al., Proceedings of the National Academies of Science, 2002, 99(4), 2392-2397.
Margolskee, The Journal of Biological Chemistry, 2002, 277(1), 1-4.
Li et al., Proceedings of the National Academies of Science, 2002, 99(7), 4692-4696.
Li X, et al., “Human Receptors for Sweet and Umami Taste”, Proc. Natl. Acad. Sci. Apr. 2, 2002, vol. 99, No. 7, pp 4692-4696.
Hoon et al. “Putative Mammalian Taste Recetprs: A Class of Taste Specific GPCRs with distinct Topographic Selectivity”, Cell, Feb. 19, 2004, vol. 96, pp 541-551.
Wu et al; “Expression of Bitter Taste Receptors of the T2R family in Gastrointestinal Tract and Enteroendocrine STC-1 Cells”, Proc. Natl. Acad. Sci., Feb. 19, 2002, vol. 99 No. 4, pp 2392-2397.
Noar et al. “Activation of MAPK Cascades by G-Protein Coupled Receptors: The Case of Gonadotropin-Releasing Hormone Receptor”, Trends in Endocrinology Metabolism, 200, vol. 11, No. 3, pp 91-99.
McDonald et al, “A Scintallation Proximity Assay for the Raf-MEK/ERK Kinase Cascade: High-Throughput Screening and Identification of Selective Enzyme Inhibitors”, Analytical Biochemistry, 199, 268 pp 318-329.
Brust Paul
Ozeck Mark
Servant Guy
Xu Hong
Duane Morris LLP
Mayer Suzanne M.
Senomyx Inc.
Teskin Robin L.
LandOfFree
Functional coupling of T1Rs and T2Rs by Gi proteins, and... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Functional coupling of T1Rs and T2Rs by Gi proteins, and..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Functional coupling of T1Rs and T2Rs by Gi proteins, and... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3557105