Multicellular living organisms and unmodified parts thereof and – Method of using a plant or plant part in a breeding process... – Method of breeding involving a mutation step
Patent
1997-04-10
1999-10-05
Benzion, Gary
Multicellular living organisms and unmodified parts thereof and
Method of using a plant or plant part in a breeding process...
Method of breeding involving a mutation step
800267, 800275, 800298, 8003201, 536 241, 536 236, 536 2433, A01H 400, C12N 1509
Patent
active
059627646
ABSTRACT:
Insertions into a gene of known sequence can be generated by crossing two parent plants, one of which contains a transposable element, to produce F.sub.1 progeny plants in which the insertion is detected by means of a PCR. F.sub.1 progeny plants containing such an insertion are self-fertilized to produce F.sub.2 progeny which are homozygous for the insertion. The function of a gene disabled by the insertion can be ascertained from a comparison of the phenotype of the F.sub.2 progeny with a parental phenotype. Large numbers of F.sub.1 progeny can be tested simultaneously for the presence of insertions. A collection of F.sub.2 seed can be stored and used for phenotype comparison when an insertion is detected.
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Briggs Steven P.
Meeley Robert B.
Benzion Gary
Pioneer Hi-Bred International , Inc.
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