Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
1994-11-18
2001-12-04
Priebe, Scott D. (Department: 1632)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S007210, C435S007400, C435S007900
Reexamination Certificate
active
06326138
ABSTRACT:
FIELD OF THE INVENTION
The present invention relates to analytical methods. In a particular aspect, the present invention relates to methods for the identification of compounds which induce and/or inhibit signal transduction in cells.
BACKGROUND OF THE INVENTION
Messages are transmitted in cells (and between cells) by a process called signal transduction. Signal transduction is the means by which the presence (or occurrence) of stimuli are communicated within (or between) cells. Since communication within (and between) cells is central to the control of many biological responses, and because many stimuli promote signal transduction, the ability to rapidly monitor signal transduction in response to a given stimulus would be very useful. Such ability would be especially useful for the screening of compounds to identify those capable of modulating signal transduction.
While any viable cell can be used for such screening, especially useful are neuronal cells, which allow the study of signal transduction in the central nervous system. Long-term cell cultures can be obtained from numerous sources, e.g, immortalized cells. The application of the invention techniques, therefore, to a variety of readily available cell types, will allow fundamental questions of cellular and molecular interactions among the myriad of functionally distinct cell types which contribute to development and functioning of mammalian species to be addressed.
BRIEF DESCRIPTION OF THE INVENTION
In accordance with the present invention, we have developed a novel analytical method for identifying compounds which induce and/or inhibit signal transduction in cells. The invention method enables rapid testing of a variety of compounds to determine if they exert an influence on signal transduction. The invention assay can be carried out using unmodified cells and/or cell lines, avoiding the need for cell transformation with labor intensive constructs (e.g., reporter constructs) prior to analysis.
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Cauley Keith A.
Verma Inder M.
Foley & Lardner
Priebe Scott D.
Reiter Stephen E.
The Salk Institute for Biological Studies
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