Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-05-22
1998-04-28
Horlick, Kenneth R.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 71, 435 912, 435 691, 435 711, 4351723, C12Q 168, C12P 1934
Patent
active
057443033
ABSTRACT:
A method for determining whether an individual, i.e., a human, carries a mutation in a gene which encodes a transcriptional regulator (e.g., a tumor suppressor). The method includes the steps of obtaining a nucleic acid sample comprising cDNAs encoding both alleles of the gene from a cell sample from the individual; providing a nucleic acid construct comprising a binding site operably linked to DNA encoding a detectable protein such that transcription of the DNA encoding the detectable protein is regulated by interaction of the transcriptional regulator polypeptide with the binding site; transfecting the nucleic acid sample and the nucleic acid construct into cells with low levels of the transcriptional regulator polypeptide; and then determining the levels of the detectable protein in the transfected cells, this level being correlated with the presence of a mutation in at least one of the alleles of the gene.
REFERENCES:
patent: 4942123 (1990-07-01), Lee et al.
patent: 4981784 (1991-01-01), Evans et al.
patent: 4997757 (1991-03-01), Schiestl
patent: 5011773 (1991-04-01), Lee et al.
patent: 5183736 (1993-02-01), Pfahl et al.
patent: 5362623 (1994-11-01), Vogelstein et al.
Guarente, Cell 52, 303-305 (1988).
Guthrie et al., eds., Meth. Enzymol. 194, 373-398 (1991).
Frebowry et al., Cancer Res. 52 6976-6978 (1992).
Ishioka et al., Nature Gen. 5, 124-129 (1993).
Baker et al., Science 249:912-915 (1990), "Suppression of Human Colorectal Carcinoma Cell Growth by Wild-Type p53".
Bressac et al., Nature 350:429-431 (1991), "Selective G to T mutations of p53 gene in hepatocellular carcinoma from southern Africa".
B.o slashed.rresen et al., Cancer Research 52:3234-3236 (1992), "Screening for Germ Line TP53 Mutations in Breast Cancer Patients".
B.o slashed.rresen et al., Proc. Natl. Acad. Sci. USA 88:8405-8409 (1991), "Constant denaturant gel electrophoresis as a rapid screening technique for p53 mutations".
Cance et al., The New England Journal of Medicine 323:1457-1462 (1990), "Altered Expression of the Retino-blastoma Gene Product in Human Sarcomas".
Cotton et al., Proc. Natl. Acad. Sci. USA 85:4397-4401 (1988), "Reactivity of cytosine and thymine in single-base-pair mismatches with hydroxylamine and osmium tetroxide and its application to the study of mutations".
Dalbagni et al., Proceedings of the AACR 33:373, Abstract .pi.2229 (1992), "P53 and chromosome 17 abnormalities in human bladder tumors".
Diller et al., Molecular and Cellular Biology 10:5772-5781 (1990), "p53 Functions as a Cell Cycle Control Protein in Osteosarcomas".
El-Diery et al., Nature Genetics 1:45-49 (1992), "Definition of a consensus binding site for p53".
Farmer et al., Nature 358:83-86 (1992), "Wild-type p53 activates transcription in vitro".
Fields et al., Science 249:1046-1048 (1990), "Presence of a Potent Transcription Activating Sequence in the p53 Protein".
Frebourg et al., Proc. Natl. Acad. Sci. USA 89:6413-6417 (1992), "Germ-Line mutations of the p53 tumor suppressor gene in patients with high risk for cancer inactivate the p53 protein".
Funk et al., Molecular and Cellular Biology 12:2866-2871 (1992), "A Transcriptionally Active DNA-Binding Site for Human p53 Protein Complexes".
Hsu et al., Nature 350:427-428 (1991), "Mutational hotspot in the p53 gene in human hepatocellular carcinomas".
Iavarone et al., Proc. Natl. Acad. Sci. USA 89:4207-4209 (1992), "Germ-line and somatic p53 gene mutations in multifocal osteogenic sarcoma".
Kern et al., Science 256:827-830 (1992), "Oncogenic Forms of p53 Inhibit p53-Regulated Gene Expression".
Kern et al., Science 252:1708-1711 (1991), "Identification of p53 as a Sequence-Specific DNA-Binding Protein".
Law et al., Cancer Research 51:6385-6387 (1991), "A Germ Line Mutation in Exon 5 of the p53 Gene in an Extended Cancer Family".
Lee et al., Nature 329:642-645 (1987), "The retinoblastoma susceptibility gene encodes a nuclear phospho-protein associated with DNA binding activity".
Li et al., Journal of the National Cancer Institute 84:1156-1160 (1992), "Recommendations on Predictive Testing for Germ Line p53 Mutations Among Cancer-Prone Individuals".
Madden et al., Science 253:1550-1553 (1991), "Transcriptional Repression Mediated by the WT1 Wilms Tumor Gene product".
Malkin et al., The New Eng. J. of Medicine 326:1309-1315 (1992), "Germline Mutations of the p53 Tumor-Suppressor Gene in Children and Young Adults with Second Malignant Neoplasms".
Malkin et al., Science 250:1233-1238 (1990), "Germ Line p53 Mutations in a Familial Syndrome of Breast Cancer, Sarcomas, and Other Neoplasms".
Metzger et al., Proc. Natl. Acad. Sci. USA 88:7825-7829 (1991), "Identification of a germ-line mutation in the p53 gene in a patient with an intracranial ependymoma".
Raycroft et al., Science 249:1049-1051 (1990), "Transcriptional Activation by Wild-Type But Not Transforming Mutants of the p53 Anti-Oncogene".
Romano et al., Oncogene 4:1483-1488 (1989), "Identification and characterization of a p53 gene mutation in a human osteosarcoma cell line".
Santibanez-Korff et al., The Lancet 338:1490-1491 (1991), "p53 germline mutations in Li-Fraumeni syndrome".
Sarkis et al., Proc. Am. Assoc. Cancer Res. 33:254 (1992), "p53 Mutations as a Predictor of Biological Behavior of T1 Bladder Carcinoma".
Sidransky et al., Cancer Research 52:2984-2986 (1992), "Inherited p53 Gene Mutations in Breast Cancer".
Srivastava et al., Nature 348:747-749 (1990), "Germ-line transmission of a mutated p53 gene in a cancer-prone family with Li-Fraumeni syndrome".
Thor et al., Journal of the Natl. Cancer Inst. 84:845-855, 1992 "Accumulation of p53 Tumor Suppressor Gene Protein: An Independent Marker of Prognosis in Breast Cancers".
Toguchida et al., New Engl. J. Med. 326:1301-1308 (1992).
Vogelstein, Nature 348:681-682 (1990), "A deadly inheritance".
Scharer et al., Nucleic Acids Research 20:1539-1545 (1992), "Mammalian p53 can function as a transcription factor in yeast."
Donehower et al., Nature 356:215-221 (1992), "Mice deficient for p53 are developmentally normal but susceptible to spontaneous tumours."
Hurwitz et al., The American Journal of Surgery 164:299-306 (1992), "Diagnostic and Prognostic Molecular Markers in Cancer."
Weintraub et al., Proc. Natl. Acad. Sci. USA 88:4570-4571 (1991), "The MCK enhancer contains a p53 responsive element".
Frebourg Thierry
Friend Stephen H.
Iggo Richard
Ishioka Chikashi
Horlick Kenneth R.
Institut Suisse de Recherches Experimentales
The General Hospital Corporation
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