Chemistry: molecular biology and microbiology – Process of utilizing an enzyme or micro-organism to destroy... – Treating animal or plant material or micro-organism
Patent
1997-07-07
2000-02-15
Prats, Francisco
Chemistry: molecular biology and microbiology
Process of utilizing an enzyme or micro-organism to destroy...
Treating animal or plant material or micro-organism
435262, 435197, 435135, 435136, 426 44, 426 53, 426 52, C12S 300, A23L 1015
Patent
active
060251883
ABSTRACT:
Methods for identifying organisms capable of degrading fumonisin. Fumonisin can be incorporated into culture medium for selection of organisms resistant to fumonisin and/or capable of growing on fumonisin as a sole carbon source. Using this method, several organisms have been identified. These organisms can be used to isolate the enzymes and the genes responsible for conferring fumonisin-resistance. The gene can be cloned and inserted into a suitable expression vector so that the protein can be further characterized. Additionally, the DNA encoding for fumonisin degrading enzymes can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. Methods for detoxification in grain, grain processing, silage, food crops and in animal feed and rumen microbes are also disclosed.
REFERENCES:
patent: 4988586 (1991-01-01), Toyoda et al.
patent: 5178863 (1993-01-01), Toyoda et al.
patent: 5262306 (1993-11-01), Robeson et al.
Abbas, et al. (1992) Phytotoxicity of Fumonisin B.sub.1 on Weed and Crop Species.sup.1, Weed Technology, 6:548-552.
Blackwell, et al. (1994) Production of Carbon 14-Labeled Fumonisin in Liquid Culture, Journal of AOAC International, 77(2):506-511.
Gelderblom, et al. (1993) Structure-Activity Relationships of Fumonisins in Short-Term Carcinogenesis and Cytotoxicity Assays, Food Chem. Toxic., 31(6):407-414.
Van Asch, et al. (1992) Phytotoxicity of Fumoninsin B.sub.1, Moniliformin, and T-2 Toxin to Corn Callus Cultures, Phytopathology, 82(11):1330-1332.
Vesonder, et al. (1993) Comparison of the Cytotoxicities of Fusarium Metabolites and Alternaria Metabolite AAL-Toxin to Cultured Mammalian Cell Lines, Arch. Environ. Contam, Toxicol., 24:473-477.
Tanaka, et al. (1993) Structure-Dependent Phytotoxicity of Fumonisins and Related Compounds in a Duckweed Bioassay, Phytochemistry, 33(4):779-785.
He P., et al. (1992) Microbial Transformation of Deoxynivalenol (Vomitoxin), Applied and Environmental Microbiology, 58(12):3857-3863.
Kneusel, et al. (1994) Molecular Characterization and Cloning of an Esterase Which Inactivates the Macrolide Toxin Brefeldin A*, The Journal of Biological Chemistry, 269(5):3449-3456.
Miller, J. D., et al. (1986) Degradation of deoxynivalenol by suspension cultures of the fusarium head blight resistant wheat cultivar Frontana, Canadian Journal of Plant Pathology, 8:147-150.
Ueno, et al. (1983) Metabolism of T-2 Toxin in Curtobacterium sp. Strain 114-2, Applied and Environmental Microbiology, 46:120-127.
Utsumi, et al. (1991) Molecular Cloning and Characterization of the Fusaric Acid-resistance Gene from Pseudomonas cepacia, Agric. Biol. Chem., 55:1913-1918.
Vesonder, et al. (1992) Comparative Phytotoxicity of the Fumonisins, AAL-Toxin and Yeast Sphingolipids in Lemna minor L. (Duckweed), Arch. Environ. Contam. Toxicol., 23:464-467.
Marth, et al. (1978) Update on molds: degradation of aflatoxin, J. Food Technol., 33:81-87.
Kneusel, et al. (1990) Detoxification of the macrolide toxin brefeldin A by Bacillus subtilis, FEBS Letters, 275(1-2):107-110.
Toyoda, et al. (1988) Detoxification of Fusaric Acid by Fusaric Acid-Resistant Mutant of Pseudomonas solanacearum and its Application to Biological Control of Fusarium Wilt of Tomato, Phytopathology, 78(10);1307-1311.
Bunz et al. (1993) Purification of two isosfunctional hydrolases (EC 3.7.1.8) in the degradative pathway for dibenzofuran in Sphingomonas sp. strain RW1, Biodegradation, 4:171-178.
Duvick et al. (1992) Purification and Characterization of a Novel Antimicrobial Peptide from Maize (Zea mays L.) Kernels*, The J. of Biol. Chem., 267(26):18814-18820.
Kraus et al. (1992) Synthesis fo Analogs of Fumonisin B1, J. of Agri. and Food Chem., 40(12):2331-2332.
Lotti et al. (1993) Cloning and analysis of Candida cylindracea lipase sequences, Gene, 124:45-55.
Cygler et al. (1993) Relationship between sequence conservation and three-dimensional structure in a large family of esterases, lipases, and related proteins, Protein Science, 2:366-382.
Arpagaus et al. (1991) Use of the Polymerase Chain Reaction for Homology Probing of Butyrylcholinesterase from Several Vertebrates, The J. of Biol. Chem., 266(11):6966-6974.
Van Asch et al. (1992) Phytotoxicity of Fumonisin B1, Moniliformin, and T-2 Toxin to Corn Callus Cultures, Phytopathology, 82:1330-1332.
Lagu et al., Synthesis of Fumonisin Analogs, Abstracts of Papers (Part 2), 204.sup.th American Chemical Society National Meeting, Washington, D.C., USA , (Aug. 23-28, 1992).
Zeiss, Hans-Joachim (1991) Enantioselective Synthesis of Both Enantiomers of Phosphinothricin via Asymmetric Hydrogenation of .alpha.-Acylamido Acrylates, J. Org. Chem., 56(5):1783-1788.
Bowen Benjamin A.
Duvick Jonathan
Gilliam Jacob T.
Maddox Joyce R.
Rood Tracy A.
Pioneer Hi-Bred International , Inc.
Prats Francisco
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