Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1997-03-10
1998-03-24
Wax, Robert A.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435193, 435823, C12P 1918, C12N 910, C12N 100
Patent
active
057311730
ABSTRACT:
Extracellular fructosyltransferase of Acetobacter diazotrophicus was isolated and purified and its enzymatic properties were established. Cloning, sequencing and genetic manipulation of the fructosyltransferase gene so as to produce high levels of the enzyme in recombinant prokaryotic and eukaryotic cells. Both natural and recombinant fructosyltransferase of Acetobacter diazotrophicus produce fructose-containing oligosaccharides and fructans. The enzyme yields in particular high levels of fructooligosaccharides from sucrose, such as kestose and kestotetraose which can be used as natural low-calorie sweeteners.
REFERENCES:
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Shimotsu, H. et al., "Modulation Bacillus Subtilis Levansucrase Gene Expression by Sucrose and Regulation of the Steady-State mRNA Level by sacU and sacQ Genes," Journal of Bacteriology, 168, 380-388 (1986).
Tang, L. et al., "Isolation and Characterization of Levansucrase-Encoding Gene from Bacillus Amyloliquefaciens," Gene, 96, 89-93 (1990).
Song, K. et al., "Nucleotide Sequence of Levansucrase Gene (levU) of Zymomonas Mobilis ZM1 (ATCC10988)," Biochimica et Biophysica Acta, 1173, 320-324 (1993).
Hernandez, L. et al., "Isolation and Enzymic Properties of Levansucrase Secreted by Acetobacter Diazotrophicus SRT4, a Bacterium Associated with Sugar Cane," Biochemical Journal, 309, 113-118 (1995).
Kyono, K. et al., "Cloning and Characterization of Zymomonas Mobilis Genes Encoding Extracellular Levansucrase and Invertase," Biosci. Biotech. Biochem., 59 (2), 289-293 (1995).
Arrieta, J. et al., "Molecular Characterization of the Levansucrase Gene From the Endophytic Sugarcane Bacterium Acetobacter Diazotrophicus SRT4," Microbiology, 142, 1077-1085 (1996).
Garcia Lazaro Hernandez
Gonzalez Alberto Coego
Sosa Guillermo Selman-Housein
Sosa Juan Gabriel Arrieta
Center for Genetic Engineering and Biotechnology
Hobbs Lisa J.
Wax Robert A.
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