Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease
Patent
1994-12-22
1997-06-24
Wax, Robert A.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Transferase other than ribonuclease
4352523, 43525233, 43525411, 43525423, 4353201, 536 232, C12N 910, C12N 120, C12N 114, C07H 2104
Patent
active
056416679
ABSTRACT:
Extracellular fructosyltransferase of Acetobacter diazotrophicus was isolated and purified and its enzymatic properties were established. Cloning, sequencing and genetic manipulation of the fructosyltransferase gene so as to produce high levels of the enzyme in recombinant prokaryotic and eukaryotic cells. Both natural and recombinant fructosyltransferase of Acetobacter diazotrophicus produce fructose-containing oligosaccharides and fructans. The enzyme yields in particular high levels of fructooligosaccharides from sucrose, such as kestose and kestotetraose which can be used as natural low-calorie sweeteners.
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Garcia Lazaro Hernandez
Gonzalez Alberto Coego
Sosa Guillermo Selman-Housein
Sosa Juan Gabriel Arrieta
Center for Genetic Engineering and Biotechnology
Hobbs Lisa J.
Wax Robert A.
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